PDBsum entry 2vl8

Toxin

PDB id

2vl8

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Contents

			Protein chains

					539 a.a. *

			Ligands

					UDP ×3


					CTS ×3

			Metals

					_CA ×5

			Waters ×400

* Residue conservation analysis

PDB id:

2vl8

Links

Name:

Toxin

Title:

Crystal structure of the catalytic domain of lethal toxin from clostridium sordellii in complex with udp, castanospermine and calcium ion

Structure:

Cytotoxin l. Chain: a, b, c. Fragment: catalytic domain, residues 1-546. Engineered: yes. Mutation: yes

Source:

Clostridium sordellii. Organism_taxid: 1505. Strain: 6018. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.31Å

R-factor:

0.218

R-free:

0.255

Authors:

T.Jank,M.O.P.Ziegler,G.E.Schulz,K.Aktories

Key ref:

T.Jank et al. (2008). Inhibition of the glucosyltransferase activity of clostridial Rho/Ras-glucosylating toxins by castanospermine. Febs Lett, 582, 2277-2282. PubMed id: 18505687 DOI: 10.1016/j.febslet.2008.05.025

Date:

09-Jan-08

Release date:

17-Jun-08

PROCHECK

	Headers

	References

Protein chains

Q46342 (TCSL1_PARSO) - Cytotoxin-L from Paraclostridium sordellii

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:					2364 a.a. 539 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 2 residue positions (black crosses)



		Enzyme reactions

Enzyme class 1:

E.C.2.4.1.- - ?????

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Enzyme class 2:

E.C.3.4.22.- - ?????

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

DOI no: 10.1016/j.febslet.2008.05.025	Febs Lett 582:2277-2282 (2008)
PubMed id: 18505687

Inhibition of the glucosyltransferase activity of clostridial Rho/Ras-glucosylating toxins by castanospermine.
T.Jank, M.O.Ziegler, G.E.Schulz, K.Aktories.

ABSTRACT

Castanospermine was identified as an inhibitor of the Rho/Ras-glucosylating Clostridium sordellii lethal toxin and Clostridium difficile toxin B. Microinjection of castanospermine into embryonic bovine lung cells prevented the cytotoxic effects of toxins. The crystal structure of the glucosyltransferase domain of C. sordellii lethal toxin in complex with castanospermine, UDP and a calcium ion was solved at a resolution of 2.3A. The inhibitor binds in a conformation that brings its four hydroxyl groups and its N-atom almost exactly in the positions of the four hydroxyls and of the ring oxygen of the glucosyl moiety of UDP-glucose, respectively.

Literature references that cite this PDB file's key reference

PubMed id

Reference

21308451

N.Suwantarat, and D.A.Bobak (2011).
Current Status of Nonantibiotic and Adjunct Therapies for Clostridium difficile Infection.

Curr Infect Dis Rep, 13, 21-27.

20714463

D.J.Wardrop, and S.L.Waidyarachchi (2010).
Synthesis and biological activity of naturally occurring α-glucosidase inhibitors.

Nat Prod Rep, 27, 1431-1468.

19340897

P.Hookman, and J.S.Barkin (2009).
Clostridium difficile associated infection, diarrhea and colitis.

World J Gastroenterol, 15, 1554-1580.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.