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PDBsum entry 2v52
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Structural protein/contractile protein
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PDB id
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2v52
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Contents |
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* Residue conservation analysis
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PDB id:
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Structural protein/contractile protein
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Title:
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Structure of mal-rpel2 complexed to g-actin
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Structure:
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Actin, alpha skeletal muscle. Chain: b. Synonym: actin, alpha skeletal muscle, alpha-actin-1. Mkl/myocardin-like protein 1. Chain: m. Fragment: rpel2. Synonym: mrtf-a, mal, megakaryoblastic leukemia 1 protein homolog, basic sap coiled-coil transcription activator. Engineered: yes
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Source:
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Oryctolagus cuniculus. Rabbit. Organism_taxid: 9986. Synthetic: yes. Mus musculus. Mouse. Organism_taxid: 10090
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Resolution:
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1.45Å
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R-factor:
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0.149
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R-free:
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0.188
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Authors:
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S.Mouilleron,S.Guettler,C.A.Langer,R.Treisman,N.Q.Mcdonald
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Key ref:
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S.Mouilleron
et al.
(2008).
Molecular basis for G-actin binding to RPEL motifs from the serum response factor coactivator MAL.
Embo J,
27,
3198-3208.
PubMed id:
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Date:
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01-Oct-08
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Release date:
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25-Nov-08
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PROCHECK
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Headers
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References
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Enzyme class 1:
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Chain B:
E.C.3.6.4.-
- ?????
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Enzyme class 2:
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Chain M:
E.C.?
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Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
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Embo J
27:3198-3208
(2008)
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PubMed id:
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Molecular basis for G-actin binding to RPEL motifs from the serum response factor coactivator MAL.
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S.Mouilleron,
S.Guettler,
C.A.Langer,
R.Treisman,
N.Q.McDonald.
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ABSTRACT
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Serum response factor transcriptional activity is controlled through
interactions with regulatory cofactors such as the coactivator MAL/MRTF-A
(myocardin-related transcription factor A). MAL is itself regulated in vivo by
changes in cellular actin dynamics, which alter its interaction with G-actin.
The G-actin-sensing mechanism of MAL/MRTF-A resides in its N-terminal domain,
which consists of three tandem RPEL repeats. We describe the first molecular
insights into RPEL function obtained from structures of two independent
RPEL(MAL) peptide:G-actin complexes. Both RPEL peptides bind to the G-actin
hydrophobic cleft and to subdomain 3. These RPEL(MAL):G-actin structures explain
the sequence conservation defining the RPEL motif, including the invariant
arginine. Characterisation of the RPEL(MAL):G-actin interaction by fluorescence
anisotropy and cell reporter-based assays validates the significance of
actin-binding residues for proper MAL localisation and regulation in vivo. We
identify important differences in G-actin engagement between the two RPEL(MAL)
structures. Comparison with other actin-binding proteins reveals an unexpected
similarity to the vitamin-D-binding protein, extending the G-actin-binding
protein repertoire.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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B.Visegrády,
and
L.M.Machesky
(2010).
Myopathy-causing actin mutations promote defects in serum-response factor signalling.
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Biochem J,
427,
41-48.
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E.N.Olson,
and
A.Nordheim
(2010).
Linking actin dynamics and gene transcription to drive cellular motile functions.
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Nat Rev Mol Cell Biol,
11,
353-365.
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K.Amano,
T.Yoshidome,
M.Iwaki,
M.Suzuki,
and
M.Kinoshita
(2010).
Entropic potential field formed for a linear-motor protein near a filament: Statistical-mechanical analyses using simple models.
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J Chem Phys,
133,
045103.
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L.Jin,
Q.Gan,
B.J.Zieba,
S.M.Goicoechea,
G.K.Owens,
C.A.Otey,
and
A.V.Somlyo
(2010).
The actin associated protein palladin is important for the early smooth muscle cell differentiation.
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PLoS One,
5,
e12823.
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L.Leitner,
D.Shaposhnikov,
A.Descot,
R.Hoffmann,
and
G.Posern
(2010).
Epithelial Protein Lost in Neoplasm alpha (Eplin-alpha) is transcriptionally regulated by G-actin and MAL/MRTF coactivators.
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Mol Cancer,
9,
60.
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M.W.Chan,
F.Chaudary,
W.Lee,
J.W.Copeland,
and
C.A.McCulloch
(2010).
Force-induced myofibroblast differentiation through collagen receptors is dependent on mammalian diaphanous (mDia).
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J Biol Chem,
285,
9273-9281.
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R.Pawłowski,
E.K.Rajakylä,
M.K.Vartiainen,
and
R.Treisman
(2010).
An actin-regulated importin α/β-dependent extended bipartite NLS directs nuclear import of MRTF-A.
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EMBO J,
29,
3448-3458.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
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Links
