PDBsum entry 2uut

Hydrolase

PDB id: 2uut

Contents

Protein chain

492 a.a. *

Waters ×294

* Residue conservation analysis

PDB id:

2uut

Name:

Hydrolase

Title:

The 2.4 angstrom resolution structure of the d346g mutant of the sapporo virus rdrp polymerase

Structure:

RNA-directed RNA polymerase. Chain: a. Fragment: RNA polymerase, residues 1135-1511,1518-1630,1632-1649. Synonym: sapovirus RNA-dependant RNA-polymerase. Engineered: yes. Mutation: yes

Source:

Sapporo virus. Organism_taxid: 95342. Strain: sapporo. Variant: dresden isolate. Tissue: intestine. Expressed in: escherichia coli. Expression_system_taxid: 469008. Other_details: from dresden calici-laboratory

Resolution:

2.40Å R-factor: 0.200 R-free: 0.256

Authors:

S.W.B.Fullerton,I.Robel,L.Schuldt,J.Gebhardt,P.Tucker,J.Rohayem

Key ref:

S.W.B.Fullerton et al. The 2.4 angstrom resolution structure of the d346g mutant of the sapporo virus rdrp polymerase. To be published, .

Date:

07-Mar-07 Release date: 01-May-07

Protein chain

Q69014  (POLG_SVM93) -  Genome polyprotein (Fragment) from Sapporo virus (strain Human/United Kingdom/Manchester/1993)

Seq: 2208 a.a.

Struc: 492 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 2: E.C.2.7.7.48 - RNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
• Enzyme class 3: E.C.3.4.22.66 - calicivirin.
• Enzyme class 4: E.C.3.6.1.15 - nucleoside-triphosphate phosphatase.
• Reaction: a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + phosphate + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site