PDBsum entry 2rg9

Hydrolase, toxin

PDB id: 2rg9

Contents

Protein chains

249 a.a. *

263 a.a. *

Ligands

NAG ×4

SO4

AZI ×3

GOL ×15

Metals

_CL ×2

Waters ×317

* Residue conservation analysis

PDB id:

2rg9

Name:

Hydrolase, toxin

Title:

Crystal structure of viscum album mistletoe lectin i in native state at 1.95 a resolution, comparison of structure active site conformation in ricin and in viscumin

Structure:

Beta-galactoside-specific lectin 1 chain a isoform 1. Chain: a. Synonym: beta-galactoside-specific lectin i chain a isoform 1, mla, ml-i a, rrna n-glycosidase. Engineered: yes. Beta-galactoside-specific lectin 1 chain b. Chain: b. Synonym: beta-galactoside-specific lectin i chain b, mlb, ml-i b. Engineered: yes

Source:

Viscum album. European mistletoe. European mistletoe

Resolution:

1.95Å R-factor: 0.222 R-free: 0.239

Authors:

N.U.Karpechenko,V.I.Timofeev,A.G.Gabdoulkhakov,A.M.Mikhailov

Key ref:

N.U.Karpechenko et al. Crystal structure of viscum album mistletoe lectin i in native state at 1.95 a resolution, Comparison of structure active site conformation in ricin and in viscumin. To be published, .

Date:

03-Oct-07 Release date: 07-Oct-08

Protein chain

P81446  (ML1_VISAL) -  Beta-galactoside-specific lectin 1 from Viscum album

Seq: 564 a.a.

Struc: 249 a.a.*

Protein chain

P81446  (ML1_VISAL) -  Beta-galactoside-specific lectin 1 from Viscum album

Seq: 564 a.a.

Struc: 263 a.a.*

* PDB and UniProt seqs differ at 29 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B: E.C.3.2.2.22 - rRNA N-glycosylase.
• Reaction: Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.