PDBsum entry 2pi7

Hydrolase

PDB id: 2pi7

Contents

Protein chains

294 a.a. *

Ligands

NO3 ×2

Waters ×182

* Residue conservation analysis

PDB id:

2pi7

Name:

Hydrolase

Title:

Structure of the catalytic domain of the chick retinal neurite inhibitor-receptor protein tyrosine phosphatase cryp-2/cptpro

Structure:

Protein tyrosine phosphatase cryp-2. Chain: a, b. Fragment: catalytic domain, residues 956-1267. Engineered: yes

Source:

Gallus gallus. Chicken. Organism_taxid: 9031. Gene: cryp2. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.59Å R-factor: 0.227 R-free: 0.287

Authors:

T.S.Girish,B.Gopal

Key ref:

T.S.Girish and B.Gopal (2007). The crystal structure of the catalytic domain of the chick retinal neurite inhibitor-receptor protein tyrosine phosphatase CRYP-2/cPTPRO. Proteins, 68, 1011-1015. PubMed id: 17546659 DOI: 10.1002/prot.21424

Date:

13-Apr-07 Release date: 01-May-07

Protein chains

Q98945  (Q98945_CHICK) -  protein-tyrosine-phosphatase from Gallus gallus

Seq: 1267 a.a.

Struc: 294 a.a.

Enzyme reactions

• Enzyme class: E.C.3.1.3.48 - protein-tyrosine-phosphatase.
• Reaction: O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

DOI no: 10.1002/prot.21424

Proteins 68:1011-1015 (2007)

PubMed id: 17546659

The crystal structure of the catalytic domain of the chick retinal neurite inhibitor-receptor protein tyrosine phosphatase CRYP-2/cPTPRO.

T.S.Girish, B.Gopal.

ABSTRACT

No abstract given.

Selected figure(s)

Figure 1.
Figure 1. (a) Sequence alignment of PTP D1 domains of CRYP-2, RPTP- and RPTP- , and PTP1B. The secondary structure elements corresponding to CRYP-2 are shown. A grey background has been used to highlight residues involved in oligomerization. These are poorly conserved. (b) The quaternary arrangement of CRYP-2 showing the location of the two active sites. The active site of CRYP-2 (highlighted by the red circle) is not occluded by oligomerization. The active site cysteine, aspartate, proline, tryptophan, and the bound nitrate ion are depicted in ball and stick representation. (c) Both open and closed conformations of the active site lid are seen in the crystal structure. In this superposition, the red ribbon representation is for CRYP-2 while the superposed PTP1B conformation is seen in grey. (d and e) The active site of CRYP-2 contains a nitrate ion in both the closed and open conformations. Panels d (closed conformation) and e (open conformation) show the (2Fo-Fc) electron density map calculated at 1.2 for the residues that surround the active site as well as the bound nitrate ion.

The above figure is reprinted by permission from John Wiley & Sons, Inc.: Proteins (2007, 68, 1011-1015) copyright 2007.