PDBsum entry 2okt

Lyase

PDB id: 2okt

Contents

Protein chain

342 a.a. *

Waters ×590

* Residue conservation analysis

PDB id:

2okt

Name:

Lyase

Title:

Crystal structure of o-succinylbenzoic acid synthetase from staphylococcus aureus, ligand-free form

Structure:

O-succinylbenzoic acid synthetase. Chain: a. Synonym: osb synthetase. Engineered: yes

Source:

Staphylococcus aureus subsp. Aureus. Organism_taxid: 93062. Strain: col. Gene: menc. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.30Å R-factor: 0.139 R-free: 0.181

Authors:

Y.Patskovsky,R.Toro,V.Malashkevich,J.M.Sauder,S.Ozyurt,D.Smith, M.Dickey,M.Maletic,A.Powell,T.Gheyi,S.R.Wasserman,J.Gerlt, S.K.Burley,S.C.Almo,New York Sgx Research Center For Structural Genomics (Nysgxrc)

Key ref:

D.Odokonyero et al. (2014). Loss of quaternary structure is associated with rapid sequence divergence in the OSBS family. Proc Natl Acad Sci U S A, 111, 8535-8540. PubMed id: 24872444 DOI: 10.1073/pnas.1318703111

Date:

17-Jan-07 Release date: 30-Jan-07

Protein chain

A0A0H2WWB5  (MENC_STAAC) -  o-succinylbenzoate synthase from Staphylococcus aureus (strain COL)

Seq: 333 a.a.

Struc: 342 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.4.2.1.113 - o-succinylbenzoate synthase.
• Reaction: (1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H2O
• Cofactor: Mn(2+) or Mg(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

DOI no: 10.1073/pnas.1318703111

Proc Natl Acad Sci U S A 111:8535-8540 (2014)

PubMed id: 24872444

Loss of quaternary structure is associated with rapid sequence divergence in the OSBS family.

D.Odokonyero, A.Sakai, Y.Patskovsky, V.N.Malashkevich, A.A.Fedorov, J.B.Bonanno, E.V.Fedorov, R.Toro, R.Agarwal, C.Wang, N.D.Ozerova, W.S.Yew, J.M.Sauder, S.Swaminathan, S.K.Burley, S.C.Almo, M.E.Glasner.

ABSTRACT

No abstract given.