PDBsum entry 2odb

Protein binding

PDB id: 2odb

Contents

Protein chains

177 a.a. *

35 a.a. *

Ligands

SO4 ×3

GCP

Metals

_CL

_MG

Waters ×45

* Residue conservation analysis

PDB id:

2odb

Name:

Protein binding

Title:

The crystal structure of human cdc42 in complex with the crib domain of human p21-activated kinase 6 (pak6)

Structure:

Human cell division cycle 42 (cdc42). Chain: a. Engineered: yes. Serine/threonine-protein kinase pak 6. Chain: b. Fragment: pak6 crib domain. Synonym: p21-activated kinase 6, pak-6. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: cdc42. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Synthetic: yes

Resolution:

2.40Å R-factor: 0.205 R-free: 0.232

Authors:

E.Ugochukwu,X.Yang,J.Elkins,M.Soundararajan,A.C.W.Pike,J.Eswaran, N.Burgess,J.E.Debreczeni,M.Sundstrom,C.Arrowsmith,J.Weigelt, A.Edwards,O.Gileadi,F.Von Delft,S.Knapp,D.Doyle,Structural Genomics Consortium (Sgc)

Key ref:

X.Yang et al. The crystal structure of human cdc42 in complex with the crib domain of human p21-Activated kinase 6 (pak6). To be published, .

Date:

22-Dec-06 Release date: 30-Jan-07

Protein chain

P60953  (CDC42_HUMAN) -  Cell division control protein 42 homolog from Homo sapiens

Seq: 191 a.a.

Struc: 177 a.a.

Protein chain

Q9NQU5  (PAK6_HUMAN) -  Serine/threonine-protein kinase PAK 6 from Homo sapiens

Seq: 681 a.a.

Struc: 35 a.a.

Enzyme reactions

• Enzyme class 2: Chain A: E.C.3.6.5.2 - small monomeric GTPase.
• Reaction: GTP + H2O = GDP + phosphate + H⁺

GTP + H2O = GDP (Bound ligand (Het Group name = GCP) matches with 81.82% similarity) + phosphate + H(+)

• Enzyme class 3: Chain B: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site