PDBsum entry 2nui

Hydrolase

PDB id: 2nui

Contents

Protein chain

124 a.a. *

Ligands

BTB

Metals

_CL ×3

Waters ×155

* Residue conservation analysis

PDB id:

2nui

Name:

Hydrolase

Title:

X-ray structure of synthetic [d83a]rnase a

Structure:

Ribonuclease pancreatic. Chain: a. Synonym: rnase 1, rnase a. Engineered: yes. Mutation: yes

Source:

Synthetic: yes. Bos taurus. Organism_taxid: 9913. Other_details: synthesized using a combination of boc 'in situ neutralization' solid phase peptide synthesis and native chemical ligation.

Resolution:

1.10Å R-factor: 0.178 R-free: 0.193

Authors:

D.J.Boerema,V.A.Tereshko,S.B.H.Kent

Key ref:

D.J.Boerema et al. (2008). Total synthesis by modern chemical ligation methods and high resolution (1.1 A) X-ray structure of ribonuclease A. Biopolymers, 90, 278-286. PubMed id: 17610259 DOI: 10.1002/bip.20800

Date:

09-Nov-06 Release date: 30-Oct-07

Protein chain

P61823  (RNAS1_BOVIN) -  Ribonuclease pancreatic from Bos taurus

Seq: 150 a.a.

Struc: 124 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.4.6.1.18 - pancreatic ribonuclease.
• Reaction:
  1. an [RNA] containing cytidine + H2O = an [RNA]-3'-cytidine- 3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA]
  2. an [RNA] containing uridine + H2O = an [RNA]-3'-uridine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA]

DOI no: 10.1002/bip.20800

Biopolymers 90:278-286 (2008)

PubMed id: 17610259

Total synthesis by modern chemical ligation methods and high resolution (1.1 A) X-ray structure of ribonuclease A.

D.J.Boerema, V.A.Tereshko, S.B.Kent.

ABSTRACT

The total chemical synthesis of RNase A using modern chemical ligation methods is described, illustrating the significant advances that have been made in chemical protein synthesis since Gutte and Merrifield's pioneering preparation of RNase A in 1969. The identity of the synthetic product was confirmed through rigorous characterization, including the determination of the X-ray crystal structure to 1.1 Angstrom resolution.