PDBsum entry 2nqb

Structural protein/DNA

PDB id: 2nqb

Contents

Protein chains

98 a.a. *

81 a.a. *

106 a.a. *

95 a.a. *

86 a.a. *

DNA/RNA

Waters ×264

* Residue conservation analysis

PDB id:

2nqb

Name:

Structural protein/DNA

Title:

Drosophila nucleosome structure

Structure:

Alpha-satellite DNA. Chain: i, j. Engineered: yes. Histone h3. Chain: a, e. Engineered: yes. Histone h4. Chain: b, f. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: his3. Expressed in: escherichia coli. Expression_system_taxid: 562. Drosophila melanogaster. Fruit fly. Organism_taxid: 7227.

Resolution:

2.30Å R-factor: 0.223 R-free: 0.254

Authors:

K.Luger,S.Chakravarthy

Key ref:

S.Chakravarthy and k.luger Comparative analysis of nucleosome structures from different species.. To be published, .

Date:

30-Oct-06 Release date: 11-Sep-07

Protein chains

P02299  (H3_DROME) -  Histone H3 from Drosophila melanogaster

Seq: 136 a.a.

Struc: 98 a.a.

Protein chain

P84040  (H4_DROME) -  Histone H4 from Drosophila melanogaster

Seq: 103 a.a.

Struc: 81 a.a.

Protein chains

P84051  (H2A_DROME) -  Histone H2A from Drosophila melanogaster

Seq: 124 a.a.

Struc: 106 a.a.

Protein chains

P02283  (H2B_DROME) -  Histone H2B from Drosophila melanogaster

Seq: 123 a.a.

Struc: 95 a.a.*

Protein chain

P84040  (H4_DROME) -  Histone H4 from Drosophila melanogaster

Seq: 103 a.a.

Struc: 86 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

A-T-C-A-A-T-A-T-C-C-A-C-C-T-G-C-A-G-A-T-T-C-T-A-C-C-A-A-A-A-G-T-G-T-A-T-T-T-G- 146 bases

A-T-C-A-A-T-A-T-C-C-A-C-C-T-G-C-A-G-A-T-T-C-T-A-C-C-A-A-A-A-G-T-G-T-A-T-T-T-G- 146 bases