PDBsum entry 2nnb

Oxidoreductase

PDB id: 2nnb

Contents

Protein chains

441 a.a. *

Ligands

HEM ×2

Waters ×800

* Residue conservation analysis

PDB id:

2nnb

Name:

Oxidoreductase

Title:

The q403k mutant heme domain of flavocytochrome p450 bm3

Structure:

Bifunctional p-450:nadph-p450 reductase. Chain: a, b. Fragment: heme domain. Synonym: cytochrome p450(bm-3), p450bm-3. Engineered: yes. Mutation: yes

Source:

Bacillus megaterium. Organism_taxid: 1404. Gene: cyp102a21. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.90Å R-factor: 0.185 R-free: 0.233

Authors:

J.P.Clark,J.L.R.Anderson,C.S.Miles,C.G.Mowat,G.A.Reid,S.K.Chapman

Date:

24-Oct-06 Release date: 11-Sep-07

Protein chains

P14779  (CPXB_PRIM2) -  Bifunctional cytochrome P450/NADPH--P450 reductase from Priestia megaterium (strain ATCC 14581 / DSM 32 / CCUG 1817 / JCM 2506 / NBRC 15308 / NCIMB 9376 / NCTC 10342 / NRRL B-14308 / VKM B-512 / Ford 19)

Seq: 1049 a.a.

Struc: 441 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 2: E.C.1.14.14.1 - unspecific monooxygenase.
• Reaction: an organic molecule + reduced [NADPH--hemoprotein reductase] + O2 = an alcohol + oxidized [NADPH--hemoprotein reductase] + H2O + H⁺
• Cofactor: Heme-thiolate
• Enzyme class 3: E.C.1.6.2.4 - NADPH--hemoprotein reductase.
• Reaction: 2 oxidized [cytochrome P450] + NADPH = 2 reduced [cytochrome P450] + NADP⁺ + H⁺
• Cofactor: FAD; FMN

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site