PDBsum entry 2n52

Hydrolase inhibitor

PDB id: 2n52

Contents

Protein chain

57 a.a.

PDB id:

2n52

Name:

Hydrolase inhibitor

Title:

The solution structure of the kallikrein inhibitor spink6

Structure:

Serine protease inhibitor kazal-type 6. Chain: a. Fragment: kazal-like domain residues 21-80. Synonym: kallikrein inhibitor. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: spink6, unq844/pro1782. Expressed in: escherichia coli. Expression_system_taxid: 562.

NMR struc:

20 models

Authors:

J.Grotzinger

Key ref:

S.Jung et al. (2016). The solution structure of the kallikrein-related peptidases inhibitor SPINK6. Biochem Biophys Res Commun, 471, 103-108. PubMed id: 26828269 DOI: 10.1016/j.bbrc.2016.01.172

Date:

06-Jul-15 Release date: 16-Mar-16

Protein chain

Q6UWN8  (ISK6_HUMAN) -  Serine protease inhibitor Kazal-type 6 from Homo sapiens

Seq: 80 a.a.

Struc: 57 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DOI no: 10.1016/j.bbrc.2016.01.172

Biochem Biophys Res Commun 471:103-108 (2016)

PubMed id: 26828269

The solution structure of the kallikrein-related peptidases inhibitor SPINK6.

S.Jung, J.Fischer, B.Spudy, T.Kerkow, F.D.Sönnichsen, L.Xue, A.M.Bonvin, P.Goettig, V.Magdolen, U.Meyer-Hoffert, J.Grötzinger.

ABSTRACT

Kallikrein-related peptidases (KLKs) are crucial for epidermal barrier function and are involved in the proteolytic regulation of the desquamation process. Elevated KLK levels were reported in atopic dermatitis. In skin, the proteolytic activity of KLKs is regulated by specific inhibitors of the serine protease inhibitor of Kazal-type (SPINK) family. SPINK6 was shown to be expressed in human stratum corneum and is able to inhibit several KLKs such as KLK4, -5, -12, -13 and -14. In order to understand the structural traits of the specific inhibition we solved the structure of SPINK6 in solution by NMR-spectroscopy and studied its interaction with KLKs. Thereby, beside the conserved binding mode, we identified an alternate binding mode which has so far not been observed for SPINK inhibitors.