PDBsum entry 2mp8

Antimicrobial protein

PDB id: 2mp8

Contents

Protein chain

64 a.a.

PDB id:

2mp8

Name:

Antimicrobial protein

Title:

Nmr structure of nkr-5-3b

Structure:

Nkr-5-3b. Chain: a

Source:

Enterococcus faecalis. Organism_taxid: 1351. Strain: nkr-5-3

NMR struc:

20 models

Authors:

K.J.Rosengren,D.J.Craik

Key ref:

K.Himeno et al. (2015). Identification, Characterization, and Three-Dimensional Structure of the Novel Circular Bacteriocin, Enterocin NKR-5-3B, from Enterococcus faecium. Biochemistry, 54, 4863-4876. PubMed id: 26174911 DOI: 10.1021/acs.biochem.5b00196

Date:

13-May-14 Release date: 13-May-15

Protein chain

A0A0M3KKS4  (A0A0M3KKS4_ENTFL) -  Nkr-5-3b from Enterococcus faecalis

Seq: 64 a.a.

Struc: 64 a.a.

DOI no: 10.1021/acs.biochem.5b00196

Biochemistry 54:4863-4876 (2015)

PubMed id: 26174911

Identification, Characterization, and Three-Dimensional Structure of the Novel Circular Bacteriocin, Enterocin NKR-5-3B, from Enterococcus faecium.

K.Himeno, K.J.Rosengren, T.Inoue, R.H.Perez, M.L.Colgrave, H.S.Lee, L.Y.Chan, S.T.Henriques, K.Fujita, N.Ishibashi, T.Zendo, P.Wilaipun, J.Nakayama, V.Leelawatcharamas, H.Jikuya, D.J.Craik, K.Sonomoto.

ABSTRACT

Enterocin NKR-5-3B, one of the multiple bacteriocins produced by Enterococcus faecium NKR-5-3, is a 64-amino acid novel circular bacteriocin that displays broad-spectrum antimicrobial activity. Here we report the identification, characterization, and three-dimensional nuclear magnetic resonance solution structure determination of enterocin NKR-5-3B. Enterocin NKR-5-3B is characterized by four helical segments that enclose a compact hydrophobic core, which together with its circular backbone impart high stability and structural integrity. We also report the corresponding structural gene, enkB, that encodes an 87-amino acid precursor peptide that undergoes a yet to be described enzymatic processing that involves adjacent cleavage and ligation of Leu(24) and Trp(87) to yield the mature (circular) enterocin NKR-5-3B.