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PDBsum entry 2kig
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Endocytosis, hydrolase PDB id
2kig

 

 

 

 

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Contents
Protein chain
161 a.a.
PDB id:
2kig
Name: Endocytosis, hydrolase
Title: A ph domain within ocrl bridges clathrin mediated membrane trafficking to phosphoinositide metabolism
Structure: Inositol polyphosphate 5-phosphatase ii isoform. Chain: a. Fragment: unp residues 1-156. Engineered: yes
Source: Mus musculus. Mouse. Organism_taxid: 10090. Gene: inpp5b. Expressed in: escherichia coli. Expression_system_taxid: 562.
NMR struc: 20 models
Authors: Y.Mao,M.E.Hodsdon,P.De Camilli
Key ref: Y.Mao et al. (2009). A PH domain within OCRL bridges clathrin-mediated membrane trafficking to phosphoinositide metabolism. Embo J, 28, 1831-1842. PubMed id: 19536138
Date:
03-May-09     Release date:   30-Jun-09    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q8K337  (I5P2_MOUSE) -  Type II inositol 1,4,5-trisphosphate 5-phosphatase from Mus musculus
Seq:
Struc: 		 
Seq:
Struc: 		993 a.a.
161 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.3.1.3.36  - phosphoinositide 5-phosphatase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway: 		1-Phosphatidyl-myo-inositol Metabolism
      Reaction: a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + phosphate
1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)
 + H2O
 = 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)
 + phosphate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 
    Key reference    
 
 
Embo J 28:1831-1842 (2009)
PubMed id: 19536138  
 
 
A PH domain within OCRL bridges clathrin-mediated membrane trafficking to phosphoinositide metabolism.
Y.Mao, D.M.Balkin, R.Zoncu, K.S.Erdmann, L.Tomasini, F.Hu, M.M.Jin, M.E.Hodsdon, P.De Camilli.
 
  ABSTRACT  
 
OCRL, whose mutations are responsible for Lowe syndrome and Dent disease, and INPP5B are two similar proteins comprising a central inositol 5-phosphatase domain followed by an ASH and a RhoGAP-like domain. Their divergent NH2-terminal portions remain uncharacterized. We show that the NH2-terminal region of OCRL, but not of INPP5B, binds clathrin heavy chain. OCRL, which in contrast to INPP5B visits late stage endocytic clathrin-coated pits, was earlier shown to contain another binding site for clathrin in its COOH-terminal region. NMR structure determination further reveals that despite their primary sequence dissimilarity, the NH2-terminal portions of both OCRL and INPP5B contain a PH domain. The novel clathrin-binding site in OCRL maps to an unusual clathrin-box motif located in a loop of the PH domain, whose mutations reduce recruitment efficiency of OCRL to coated pits. These findings suggest an evolutionary pressure for a specialized function of OCRL in bridging phosphoinositide metabolism to clathrin-dependent membrane trafficking.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
21031565 H.Hichri, J.Rendu, N.Monnier, C.Coutton, O.Dorseuil, R.V.Poussou, G.Baujat, A.Blanchard, F.Nobili, B.Ranchin, M.Remesy, R.Salomon, V.Satre, and J.Lunardi (2011).
From Lowe syndrome to Dent disease: correlations between mutations of the OCRL1 gene and clinical and biochemical phenotypes.
  Hum Mutat, 32, 379-388.  
21666675 M.Pirruccello, L.E.Swan, E.Folta-Stogniew, and P.De Camilli (2011).
Recognition of the F&H motif by the Lowe syndrome protein OCRL.
  Nat Struct Mol Biol, 18, 789-795.
PDB code: 3qis
21378754 X.Hou, N.Hagemann, S.Schoebel, W.Blankenfeldt, R.S.Goody, K.S.Erdmann, and A.Itzen (2011).
A structural basis for Lowe syndrome caused by mutations in the Rab-binding domain of OCRL1.
  EMBO J, 30, 1659-1670.
PDB code: 3qbt
20679431 F.Nakatsu, R.M.Perera, L.Lucast, R.Zoncu, J.Domin, F.B.Gertler, D.Toomre, and P.De Camilli (2010).
The inositol 5-phosphatase SHIP2 regulates endocytic clathrin-coated pit dynamics.
  J Cell Biol, 190, 307-315.  
20133602 L.E.Swan, L.Tomasini, M.Pirruccello, J.Lunardi, and P.De Camilli (2010).
Two closely related endocytic proteins that share a common OCRL-binding motif with APPL1.
  Proc Natl Acad Sci U S A, 107, 3511-3516.  
20872266 S.P.Bothwell, L.W.Farber, A.Hoagland, and R.L.Nussbaum (2010).
Species-specific difference in expression and splice-site choice in Inpp5b, an inositol polyphosphate 5-phosphatase paralogous to the enzyme deficient in Lowe Syndrome.
  Mamm Genome, 21, 458-466.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

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