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PDBsum entry 2jd8
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Metal transport
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PDB id
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2jd8
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Contents |
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* Residue conservation analysis
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PDB id:
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Metal transport
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Title:
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Crystal structure of the zn-soaked ferritin from the hyperthermophilic archaeal anaerobe pyrococcus furiosus
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Structure:
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Ferritin homolog. Chain: 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, a, b, c, d, e, f, g, h, i, j, k, l, m, n, o, p, q, r, s, t, u, v, w, x, y, z. Synonym: ferritin. Engineered: yes
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Source:
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Pyrococcus furiosus. Organism_taxid: 2261. Expressed in: escherichia coli. Expression_system_taxid: 562
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Resolution:
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2.80Å
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R-factor:
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0.203
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R-free:
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0.251
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Authors:
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J.Tatur,W.R.Hagen,P.M.Matias
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Key ref:
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J.Tatur
et al.
(2007).
Crystal structure of the ferritin from the hyperthermophilic archaeal anaerobe Pyrococcus furiosus.
J Biol Inorg Chem,
12,
615-630.
PubMed id:
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Date:
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05-Jan-07
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Release date:
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27-Feb-07
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PROCHECK
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Headers
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References
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Q8U2T8
(Q8U2T8_PYRFU) -
Ferritin homolog from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
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Seq:
Struc:
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174 a.a.
167 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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J Biol Inorg Chem
12:615-630
(2007)
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PubMed id:
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Crystal structure of the ferritin from the hyperthermophilic archaeal anaerobe Pyrococcus furiosus.
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J.Tatur,
W.R.Hagen,
P.M.Matias.
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ABSTRACT
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The crystal structure of the ferritin from the archaeon, hyperthermophile and
anaerobe Pyrococcus furiosus (PfFtn) is presented. While many ferritin
structures from bacteria to mammals have been reported, until now only one was
available from archaea, the ferritin from Archaeoglobus fulgidus (AfFtn). The
PfFtn 24-mer exhibits the 432 point-group symmetry that is characteristic of
most ferritins, which suggests that the 23 symmetry found in the previously
reported AfFtn is not a common feature of archaeal ferritins. Consequently, the
four large pores that were found in AfFtn are not present in PfFtn. The
structure has been solved by molecular replacement and refined at 2.75-Angstrom
resolution to R = 0.195 and R(free) = 0.247. The ferroxidase center of the
aerobically crystallized ferritin contains one iron at site A and shows sites B
and C only upon iron or zinc soaking. Electron paramagnetic resonance studies
suggest this iron depletion of the native ferroxidase center to be a result of a
complexation of iron by the crystallization salt. The extreme thermostability of
PfFtn is compared with that of eight structurally similar ferritins and is
proposed to originate mostly from the observed high number of intrasubunit
hydrogen bonds. A preservation of the monomer fold, rather than the 24-mer
assembly, appears to be the most important factor that protects the ferritin
from inactivation by heat.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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G.Khare,
V.Gupta,
P.Nangpal,
R.K.Gupta,
N.K.Sauter,
and
A.K.Tyagi
(2011).
Ferritin structure from Mycobacterium tuberculosis: comparative study with homologues identifies extended C-terminus involved in ferroxidase activity.
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PLoS One,
6,
e18570.
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PDB code:
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P.Ceci,
E.Forte,
G.Di Cecca,
M.Fornara,
and
E.Chiancone
(2011).
The characterization of Thermotoga maritima ferritin reveals an unusual subunit dissociation behavior and efficient DNA protection from iron-mediated oxidative stress.
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Extremophiles,
15,
431-439.
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Y.Peng,
Y.Luo,
T.Yu,
X.Xu,
K.Fan,
Y.Zhao,
and
K.Yang
(2011).
A Blue Native-PAGE analysis of membrane protein complexes in Clostridium thermocellum.
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BMC Microbiol,
11,
22.
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D.L.Geiser,
M.C.Shen,
J.J.Mayo,
and
J.J.Winzerling
(2009).
Iron loaded ferritin secretion and inhibition by CI-976 in Aedes aegypti larval cells.
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Comp Biochem Physiol B Biochem Mol Biol,
152,
352-363.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
