PDBsum entry 2hw5

Lyase

PDB id: 2hw5

Contents

Protein chains

(+ 0 more) 260 a.a. *

Ligands

COO ×2

Metals

_MG ×4

* Residue conservation analysis

PDB id:

2hw5

Name:

Lyase

Title:

The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1

Structure:

Enoyl-coa hydratase. Chain: a, b, c, d, e, f. Synonym: short chain enoyl-coa hydratase, sceh, enoyl-coa hydratase 1. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: echs1. Expressed in: escherichia coli. Expression_system_taxid: 562.

Biol. unit:

Hexamer (from PQS)

Resolution:

2.55Å R-factor: 0.216 R-free: 0.252

Authors:

A.P.Turnbull,E.Salah,F.Niesen,J.Debreczeni,E.Ugochukwu,A.C.W.Pike, K.Kavanagh,O.Gileadi,F.Gorrec,C.Umeano,F.Von Delft,J.Weigelt, A.Edwards,C.Arrowsmith,M.Sundstrom,U.Oppermann,Structural Genomics Consortium (Sgc)

Key ref:

A.P.Turnbull et al. The crystal structure of human enoyl-Coenzyme a (CoA) hydratase short chain 1, Echs1. To be published, .

Date:

31-Jul-06 Release date: 22-Aug-06

Protein chains

P30084  (ECHM_HUMAN) -  Enoyl-CoA hydratase, mitochondrial from Homo sapiens

Seq: 290 a.a.

Struc: 260 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 1: E.C.4.2.1.17 - enoyl-CoA hydratase.
• Reaction:
  1. a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O
  2. a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O

(3S)-3-hydroxyacyl-CoA (Bound ligand (Het Group name = COO) matches with 64.81% similarity) = (2E)-enoyl-CoA + H2O

• Enzyme class 2: E.C.5.3.3.8 - Delta(3)-Delta(2)-enoyl-CoA isomerase.
• Reaction:
  1. a (3Z)-enoyl-CoA = a 4-saturated (2E)-enoyl-CoA
  2. a (3E)-enoyl-CoA = a 4-saturated (2E)-enoyl-CoA

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site