PDBsum entry 2hm9

Oxidoreductase/oxidoreductase inhibitor

PDB id: 2hm9

Contents

Protein chain

162 a.a. *

Ligands

TRR

* Residue conservation analysis

PDB id:

2hm9

Name:

Oxidoreductase/oxidoreductase inhibitor

Title:

Solution structure of dihydrofolate reductase complexed with trimethoprim, 33 structures

Structure:

Dihydrofolate reductase. Chain: a. Synonym: dhfr. Engineered: yes

Source:

Lactobacillus casei. Organism_taxid: 1582. Gene: fola, dhfr. Expressed in: escherichia coli. Expression_system_taxid: 562.

NMR struc:

33 models

Authors:

V.I.Polshakov,B.Birdsall

Key ref:

J.Feeney et al. (2011). NMR structures of apo L. casei dihydrofolate reductase and its complexes with trimethoprim and NADPH: contributions to positive cooperative binding from ligand-induced refolding, conformational changes, and interligand hydrophobic interactions. Biochemistry, 50, 3609-3620. PubMed id: 21410224

Date:

11-Jul-06 Release date: 05-Jun-07

Protein chain

P00381  (DYR_LACCA) -  Dihydrofolate reductase from Lacticaseibacillus casei

Seq: 163 a.a.

Struc: 162 a.a.

Enzyme reactions

• Enzyme class: E.C.1.5.1.3 - dihydrofolate reductase.
• Pathway: Folate Coenzymes
• Reaction: (6S)-5,6,7,8-tetrahydrofolate + NADP⁺ = 7,8-dihydrofolate + NADPH + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Biochemistry 50:3609-3620 (2011)

PubMed id: 21410224

NMR structures of apo L. casei dihydrofolate reductase and its complexes with trimethoprim and NADPH: contributions to positive cooperative binding from ligand-induced refolding, conformational changes, and interligand hydrophobic interactions.

J.Feeney, B.Birdsall, N.V.Kovalevskaya, Y.D.Smurnyy, E.M.Navarro Peran, V.I.Polshakov.

ABSTRACT

No abstract given.