PDBsum entry 2gc7

Oxidoreductase, electron transport

PDB id: 2gc7

Contents

Protein chains

382 a.a. *

125 a.a. *

105 a.a. *

147 a.a. *

Ligands

HEC ×4

Metals

_NA ×4

Waters ×1234

* Residue conservation analysis

PDB id:

2gc7

Name:

Oxidoreductase, electron transport

Title:

Substrate reduced, copper free complex of methylamine dehydrogenase, amicyanin and cytochrome c551i from paracoccus denitrificans.

Structure:

Methylamine dehydrogenase heavy chain. Chain: a, e, i, m. Synonym: madh. Methylamine dehydrogenase light chain. Chain: b, f, j, n. Synonym: madh. Amicyanin. Chain: c, g, k, o. CytochromE C-l.

Source:

Paracoccus denitrificans. Organism_taxid: 266. Organism_taxid: 266

Resolution:

1.90Å R-factor: 0.171 R-free: 0.198

Authors:

Z.Chen,R.Durley,V.L.Davidson,F.S.Mathews

Key ref:

Z.Chen et al. Structral comparison of the oxidized ternary electron transfer complex of methylamine dehydrogenase, Amicyanin and cytochrome c551i from paracoccus denitrificans with the substrate-Reduced, Copper free complex at 1.9 a resolution.. To be published, .

Date:

13-Mar-06 Release date: 06-Feb-07

Protein chains

P29894  (DHMH_PARDE) -  Methylamine dehydrogenase heavy chain from Paracoccus denitrificans

Seq: 417 a.a.

Struc: 382 a.a.*

Protein chains

P22619  (DHML_PARDE) -  Methylamine dehydrogenase light chain from Paracoccus denitrificans

Seq: 188 a.a.

Struc: 125 a.a.*

Protein chains

P22364  (AMCY_PARDE) -  Amicyanin from Paracoccus denitrificans

Seq: 131 a.a.

Struc: 105 a.a.

Protein chains

P29899  (CYCL_PARDE) -  Cytochrome c-L from Paracoccus denitrificans

Seq: 177 a.a.

Struc: 147 a.a.

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B, E, F, I, J, M, N: E.C.1.4.9.1 - methylamine dehydrogenase (amicyanin).
• Reaction: 2 oxidized [amicyanin] + methylamine + H2O = 2 reduced [amicyanin] + formaldehyde + NH4⁺ + 2 H⁺
• Cofactor: Tryptophan tryptophylquinone

Molecule diagrams generated from .mol files obtained from the KEGG ftp site