PDBsum entry 2f0n

Hydrolase

PDB id: 2f0n

Contents

Protein chain

136 a.a. *

Waters ×58

* Residue conservation analysis

PDB id:

2f0n

Name:

Hydrolase

Title:

Crystal structure of staphylococcal nuclease mutant l25i/i72l

Structure:

Staphylococcal nuclease. Chain: a. Synonym: thermonuclease. Tnase. Micrococcal nuclease. Engineered: yes. Mutation: yes

Source:

Staphylococcus aureus. Organism_taxid: 1280. Gene: nuc. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.70Å R-factor: 0.205 R-free: 0.280

Authors:

J.Z.Lu,J.Sakon,W.E.Stites

Key ref:

J.Z.Lu et al. Hydrophobic core mutants of staphylococcal nuclease. To be published, .

Date:

13-Nov-05 Release date: 24-Oct-06

Protein chain

P00644  (NUC_STAAU) -  Thermonuclease from Staphylococcus aureus

Seq: 231 a.a.

Struc: 136 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.1.31.1 - micrococcal nuclease.
• Reaction: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.