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PDBsum entry 2dth
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protein ligands Protein-protein interface(s) links
Ligase PDB id
2dth

 

 

 

 

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Contents
Protein chains
235 a.a. *
Ligands
ADP ×2
BTN ×2
Waters ×611
* Residue conservation analysis
PDB id:
2dth
Name: Ligase
Title: The crystal structure of the orthorhombic form of biotin protein ligase from pyrococcus horikoshii ot3 in complex with biotin and adp
Structure: 235aa long hypothetical biotin--[acetyl-coa-carboxylase] ligase. Chain: a, b. Synonym: biotin protein ligase. Engineered: yes
Source: Pyrococcus horikoshii. Organism_taxid: 70601. Strain: ot3. Gene: bira. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
1.95Å     R-factor:   0.240     R-free:   0.269
Authors: B.Bagautdinov,N.Kunishima,Riken Structural Genomics/proteomics Initiative (Rsgi)
Key ref: B.Bagautdinov and n.kunishima Ligand structures of biotin protein ligase from pyrococcus horikoshii ot3. To be published, .
Date:
12-Jul-06     Release date:   12-Jan-07    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
O57883  (O57883_PYRHO) -  235aa long hypothetical biotin--[acetyl-CoA-carboxylase] ligase from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Seq:
Struc: 		235 a.a.
235 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.6.3.4.15  - biotin--[biotin carboxyl-carrier protein] ligase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: biotin + L-lysyl-[protein] + ATP = N6-biotinyl-L-lysyl-[protein] + AMP + diphosphate + H+
biotin
 + L-lysyl-[protein]
Bound ligand (Het Group name = BTN)
corresponds exactly 		+ ATP
 = N(6)-biotinyl-L-lysyl-[protein]
Bound ligand (Het Group name = ADP)
matches with 73.91% similarity 		+ AMP
 + diphosphate
 + H(+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

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