PDBsum entry 2d5b

Isomerase

PDB id: 2d5b

Contents

Protein chain

500 a.a. *

Metals

_ZN

Waters ×399

* Residue conservation analysis

PDB id:

2d5b

Name:

Isomerase

Title:

Crystal structure of thermus thermophilus methionyl tRNA synthetase y225f mutant obtained in the presence of peg6000

Structure:

Methionyl-tRNA synthetase. Chain: a. Fragment: metrs. Synonym: methionine--tRNA ligase, metrs. Engineered: yes. Mutation: yes

Source:

Thermus thermophilus. Organism_taxid: 274. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.80Å R-factor: 0.195 R-free: 0.242

Authors:

M.Konno,R.Takeda,R.Takasaka,Y.Mori,R.Ishii,S.Yokoyama,Riken Structural Genomics/proteomics Initiative (Rsgi)

Date:

31-Oct-05 Release date: 28-Nov-06

Protein chain

P23395  (SYM_THET8) -  Methionine--tRNA ligase from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 618 a.a.

Struc: 500 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.6.1.1.10 - methionine--tRNA ligase.
• Reaction: tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site