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PDBsum entry 2bo2
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Immune system
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PDB id
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2bo2
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Contents |
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* Residue conservation analysis
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PDB id:
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Immune system
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Title:
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Egf domains 1,2,5 of human emr2, a 7-tm immune system molecule, in complex with calcium.
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Structure:
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Egf-like module containing mucin-like hormone receptor-like 2 precursor. Chain: a, b. Fragment: egf domains 1,2 and 5,residues 25-118,212-260. Synonym: egf-like module emr2. Engineered: yes
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_variant: prep4.
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Resolution:
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2.60Å
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R-factor:
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0.239
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R-free:
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0.260
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Authors:
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R.J.M.Abbott,I.Spendlove,P.Roversi,P.Teriete,V.Knott,P.A.Handford, J.M.Mcdonnell,S.M.Lea
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Key ref:
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R.J.Abbott
et al.
(2007).
Structural and functional characterization of a novel T cell receptor co-regulatory protein complex, CD97-CD55.
J Biol Chem,
282,
22023-22032.
PubMed id:
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Date:
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07-Apr-05
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Release date:
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09-Aug-06
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PROCHECK
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Headers
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References
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Q9UHX3
(AGRE2_HUMAN) -
Adhesion G protein-coupled receptor E2 from Homo sapiens
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Seq:
Struc:
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823 a.a.
140 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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J Biol Chem
282:22023-22032
(2007)
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PubMed id:
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Structural and functional characterization of a novel T cell receptor co-regulatory protein complex, CD97-CD55.
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R.J.Abbott,
I.Spendlove,
P.Roversi,
H.Fitzgibbon,
V.Knott,
P.Teriete,
J.M.McDonnell,
P.A.Handford,
S.M.Lea.
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ABSTRACT
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CD97, the archetypal member of the EGF-TM7 protein family, is constitutively
expressed on granulocytes and monocytes and rapidly up-regulated on T and B
cells following activation. The key isoform of CD97 expressed on leukocytes
binds the complement regulatory protein CD55 (also termed decay-accelerating
factor). CD97 has been shown recently to mediate co-stimulation of T cells via
CD55. Here, we demonstrate that blocking the interaction between CD55 on
monocytes and CD97 on T cells leads to inhibition of proliferation and
interferon-gamma secretion. This implies that bidirectional interactions between
CD97 and CD55 are involved in T cell regulation. Structural studies presented
here reveal the molecular basis for this activity. We have solved the structure
of EMR2, a very close homolog of CD97, using x-ray crystallography. NMR-based
chemical shift mapping of the EMR2-CD55 interaction has allowed us to generate a
model for the CD97-CD55 complex. The structure of the complex reveals that the T
cell and complement regulatory activities of CD55 occur on opposite faces of the
molecule. This suggests that CD55 might simultaneously regulate both the innate
and adaptive immune responses, and we have shown that CD55 can still regulate
complement when bound to CD97.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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S.A.Jensen,
S.Iqbal,
E.D.Lowe,
C.Redfield,
and
P.A.Handford
(2009).
Structure and interdomain interactions of a hybrid domain: a disulphide-rich module of the fibrillin/LTBP superfamily of matrix proteins.
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Structure,
17,
759-768.
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PDB code:
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J.Cordle,
S.Johnson,
J.Z.Tay,
P.Roversi,
M.B.Wilkin,
B.H.de Madrid,
H.Shimizu,
S.Jensen,
P.Whiteman,
B.Jin,
C.Redfield,
M.Baron,
S.M.Lea,
and
P.A.Handford
(2008).
A conserved face of the Jagged/Serrate DSL domain is involved in Notch trans-activation and cis-inhibition.
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Nat Struct Mol Biol,
15,
849-857.
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PDB codes:
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P.N.Lalli,
M.G.Strainic,
F.Lin,
M.E.Medof,
and
P.S.Heeger
(2007).
Decay accelerating factor can control T cell differentiation into IFN-gamma-producing effector cells via regulating local C5a-induced IL-12 production.
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J Immunol,
179,
5793-5802.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
