PDBsum entry 2at3

Transport protein

PDB id: 2at3

Contents

Protein chain

184 a.a. *

Ligands

HEM-IMD

Waters ×276

* Residue conservation analysis

PDB id:

2at3

Name:

Transport protein

Title:

1.00 a crystal structure of l123v/l133v mutant of nitrophorin 4 from rhodnius prolixus complexed with imidazole at ph 5.6

Structure:

Nitrophorin 4. Chain: x. Synonym: np4. Engineered: yes. Mutation: yes

Source:

Rhodnius prolixus. Organism_taxid: 13249. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.00Å R-factor: 0.145 R-free: 0.167

Authors:

A.M.Amoia,W.R.Montfort

Key ref:

A.M.Amoia and w.r.montfort Heme distortion in nitrophorin 4: high resolution structures of mutated positions l123V and l133V and heme altered proteins. To be published, .

Date:

24-Aug-05 Release date: 22-Aug-06

Protein chain

Q94734  (NP4_RHOPR) -  Nitrophorin-4 from Rhodnius prolixus

Seq: 205 a.a.

Struc: 184 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.1.7.6.1 - nitrite dismutase.
• Reaction: 3 nitrite + 2 H⁺ = 2 nitric oxide + nitrate + H2O
• Cofactor: Heme b

Heme b (Bound ligand (Het Group name = HEM) matches with 95.45% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site