PDBsum entry 1zgx

Hydrolase

PDB id: 1zgx

Contents

Protein chains

63 a.a. *

33 a.a. *

Ligands

SO4 ×3

Waters ×234

* Residue conservation analysis

PDB id:

1zgx

Name:

Hydrolase

Title:

Crystal structure of ribonuclease mutant

Structure:

Guanyl-specific ribonuclease sa. Chain: a. Fragment: residues 1-63. Synonym: rnase sa. Engineered: yes. Other_details: the protein is cleaved between arg63 and thr64, chains a and b represent the two parts of the same molecule. Guanyl-specific ribonuclease sa. Chain: b.

Source:

Streptomyces aureofaciens. Organism_taxid: 1894. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562

Biol. unit:

Dimer (from PQS)

Resolution:

1.13Å R-factor: 0.129 R-free: 0.164

Authors:

L.Urbanikova,J.Sevcik

Key ref:

L.Urbanikova and j.sevcik Surface mutation gln to lys changed the crystal packing. To be published, .

Date:

22-Apr-05 Release date: 08-Aug-06

Protein chain

P05798  (RNSA_KITAU) -  Guanyl-specific ribonuclease Sa from Kitasatospora aureofaciens

Seq: 96 a.a.

Struc: 63 a.a.

Protein chain

P05798  (RNSA_KITAU) -  Guanyl-specific ribonuclease Sa from Kitasatospora aureofaciens

Seq: 96 a.a.

Struc: 33 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains A, B: E.C.4.6.1.24 - ribonuclease T1.
• Reaction: [RNA] containing guanosine + H2O = an [RNA fragment]-3'-guanosine- 3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment]