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PDBsum entry 1z1c
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Contents |
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* Residue conservation analysis
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PDB id:
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Virus/DNA
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Title:
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Structural determinants of tissue tropism and in vivo pathogenicity for the parvovirus minute virus of mice
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Structure:
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5'-d( Ap Tp Cp Cp Tp Cp Tp Ap Tp Cp Ap C)-3'. Chain: b. Engineered: yes. 5'-d( Ap Cp Ap Cp Cp Ap Ap Ap A)-3'. Chain: c. Engineered: yes. Coat protein vp2. Chain: a. Engineered: yes
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Source:
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Synthetic: yes. Murine minute virus (strain mvmi). Organism_taxid: 10795. Strain: mvmi. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9.
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Resolution:
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3.50Å
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R-factor:
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0.325
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R-free:
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0.329
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Authors:
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M.Kontou,L.Govindasamy,H.J.Nam,N.Bryant,A.L.Llamas-Saiz,C.Foces- Foces,E.Hernando,M.P.Rubio,R.Mckenna,J.M.Almendral,M.Agbandje- Mckenna
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Key ref:
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M.Kontou
et al.
(2005).
Structural determinants of tissue tropism and in vivo pathogenicity for the parvovirus minute virus of mice.
J Virol,
79,
10931-10943.
PubMed id:
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Date:
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03-Mar-05
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Release date:
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06-Sep-05
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PROCHECK
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Headers
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References
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P07302
(CAPSD_MUMIM) -
Capsid protein VP1 from Murine minute virus (strain MVMi)
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Seq:
Struc:
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729 a.a.
549 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 2 residue positions (black
crosses)
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A-T-C-C-T-C-T-A-T-C-A-C
12 bases
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A-C-A-C-C-A-A-A-A
9 bases
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J Virol
79:10931-10943
(2005)
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PubMed id:
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Structural determinants of tissue tropism and in vivo pathogenicity for the parvovirus minute virus of mice.
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M.Kontou,
L.Govindasamy,
H.J.Nam,
N.Bryant,
A.L.Llamas-Saiz,
C.Foces-Foces,
E.Hernando,
M.P.Rubio,
R.McKenna,
J.M.Almendral,
M.Agbandje-McKenna.
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ABSTRACT
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Two strains of the parvovirus minute virus of mice (MVM), the immunosuppressive
(MVMi) and the prototype (MVMp) strains, display disparate in vitro tropism and
in vivo pathogenicity. We report the crystal structures of MVMp virus-like
particles (MVMp(b)) and native wild-type (wt) empty capsids (MVMp(e)),
determined and refined to 3.25 and 3.75 A resolution, respectively, and their
comparison to the structure of MVMi, also refined to 3.5 A resolution in this
study. A comparison of the MVMp(b) and MVMp(e) capsids showed their structures
to be the same, providing structural verification that some heterologously
expressed parvovirus capsids are indistinguishable from wt capsids produced in
host cells. The structures of MVMi and MVMp capsids were almost identical, but
local surface conformational differences clustered from symmetry-related capsid
proteins at three specific domains: (i) the icosahedral fivefold axis, (ii) the
"shoulder" of the protrusion at the icosahedral threefold axis, and (iii) the
area surrounding the depression at the icosahedral twofold axis. The latter two
domains contain important determinants of MVM in vitro tropism (residues 317 and
321) and forward mutation residues (residues 399, 460, 553, and 558) conferring
fibrotropism on MVMi. Furthermore, these structural differences between the MVM
strains colocalize with tropism and pathogenicity determinants mapped for other
autonomous parvovirus capsids, highlighting the importance of common parvovirus
capsid regions in the control of virus-host interactions.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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M.G.Mateu
(2011).
Virus engineering: functionalization and stabilization.
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Protein Eng Des Sel,
24,
53-63.
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B.L.Gurda,
K.N.Parent,
H.Bladek,
R.S.Sinkovits,
M.A.Dimattia,
C.Rence,
A.Castro,
R.McKenna,
N.Olson,
K.Brown,
T.S.Baker,
and
M.Agbandje-McKenna
(2010).
Human bocavirus capsid structure: insights into the structural repertoire of the parvoviridae.
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J Virol,
84,
5880-5889.
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K.N.Parent,
R.Khayat,
L.H.Tu,
M.M.Suhanovsky,
J.R.Cortines,
C.M.Teschke,
J.E.Johnson,
and
T.S.Baker
(2010).
P22 coat protein structures reveal a novel mechanism for capsid maturation: stability without auxiliary proteins or chemical crosslinks.
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Structure,
18,
390-401.
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PDB codes:
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L.Riolobos,
N.Valle,
E.Hernando,
B.Maroto,
M.Kann,
and
J.M.Almendral
(2010).
Viral oncolysis that targets Raf-1 signaling control of nuclear transport.
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J Virol,
84,
2090-2099.
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S.F.Cotmore,
S.Hafenstein,
and
P.Tattersall
(2010).
Depletion of virion-associated divalent cations induces parvovirus minute virus of mice to eject its genome in a 3'-to-5' direction from an otherwise intact viral particle.
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J Virol,
84,
1945-1956.
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C.Carrasco,
M.Douas,
R.Miranda,
M.Castellanos,
P.A.Serena,
J.L.Carrascosa,
M.G.Mateu,
M.I.Marqués,
and
P.J.de Pablo
(2009).
The capillarity of nanometric water menisci confined inside closed-geometry viral cages.
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Proc Natl Acad Sci U S A,
106,
5475-5480.
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A.López-Bueno,
J.C.Segovia,
J.A.Bueren,
M.G.O'Sullivan,
F.Wang,
P.Tattersall,
and
J.M.Almendral
(2008).
Evolution to pathogenicity of the parvovirus minute virus of mice in immunodeficient mice involves genetic heterogeneity at the capsid domain that determines tropism.
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J Virol,
82,
1195-1203.
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B.Kaufmann,
P.R.Chipman,
V.A.Kostyuchenko,
S.Modrow,
and
M.G.Rossmann
(2008).
Visualization of the externalized VP2 N termini of infectious human parvovirus B19.
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J Virol,
82,
7306-7312.
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C.Carrasco,
M.Castellanos,
P.J.de Pablo,
and
M.G.Mateu
(2008).
Manipulation of the mechanical properties of a virus by protein engineering.
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Proc Natl Acad Sci U S A,
105,
4150-4155.
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B.Kaufmann,
A.López-Bueno,
M.G.Mateu,
P.R.Chipman,
C.D.Nelson,
C.R.Parrish,
J.M.Almendral,
and
M.G.Rossmann
(2007).
Minute virus of mice, a parvovirus, in complex with the Fab fragment of a neutralizing monoclonal antibody.
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J Virol,
81,
9851-9858.
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H.J.Nam,
M.D.Lane,
E.Padron,
B.Gurda,
R.McKenna,
E.Kohlbrenner,
G.Aslanidi,
B.Byrne,
N.Muzyczka,
S.Zolotukhin,
and
M.Agbandje-McKenna
(2007).
Structure of adeno-associated virus serotype 8, a gene therapy vector.
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J Virol,
81,
12260-12271.
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PDB code:
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A.López-Bueno,
M.P.Rubio,
N.Bryant,
R.McKenna,
M.Agbandje-McKenna,
and
J.M.Almendral
(2006).
Host-selected amino acid changes at the sialic acid binding pocket of the parvovirus capsid modulate cell binding affinity and determine virulence.
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J Virol,
80,
1563-1573.
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C.Carrasco,
A.Carreira,
I.A.Schaap,
P.A.Serena,
J.Gómez-Herrero,
M.G.Mateu,
and
P.J.de Pablo
(2006).
DNA-mediated anisotropic mechanical reinforcement of a virus.
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Proc Natl Acad Sci U S A,
103,
13706-13711.
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L.Govindasamy,
E.Padron,
R.McKenna,
N.Muzyczka,
N.Kaludov,
J.A.Chiorini,
and
M.Agbandje-McKenna
(2006).
Structurally mapping the diverse phenotype of adeno-associated virus serotype 4.
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J Virol,
80,
11556-11570.
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PDB code:
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
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Links
