PDBsum entry 1xhs

Structural genomics, unknown function

PDB id: 1xhs

Contents

Protein chain

113 a.a. *

* Residue conservation analysis

PDB id:

1xhs

Name:

Structural genomics, unknown function

Title:

Solution nmr structure of protein ytfp from escherichia coli. Northeast structural genomics consortium target er111.

Structure:

Hypothetical upf0131 protein ytfp. Chain: a. Engineered: yes

Source:

Escherichia coli. Organism_taxid: 562. Gene: ytfp. Expressed in: escherichia coli. Expression_system_taxid: 562.

NMR struc:

10 models

Authors:

J.M.Aramini,Y.J.Huang,G.V.T.Swapna,R.K.Paranji,R.Xiao,R.Shastry, T.B.Acton,J.R.Cort,M.A.Kennedy,G.T.Montelione,Northeast Structural Genomics Consortium (Nesg)

Key ref:

J.M.Aramini et al. (2007). Solution NMR structure of Escherichia coli ytfP expands the structural coverage of the UPF0131 protein domain family. Proteins, 68, 789-795. PubMed id: 17523190 DOI: 10.1002/prot.21450

Date:

20-Sep-04 Release date: 04-Jan-05

Protein chain

P0AE48  (YTFP_ECOLI) -  Gamma-glutamylcyclotransferase family protein YtfP from Escherichia coli (strain K12)

Seq: 113 a.a.

Struc: 113 a.a.

Enzyme reactions

• Enzyme class: E.C.?

DOI no: 10.1002/prot.21450

Proteins 68:789-795 (2007)

PubMed id: 17523190

Solution NMR structure of Escherichia coli ytfP expands the structural coverage of the UPF0131 protein domain family.

J.M.Aramini, Y.J.Huang, G.V.Swapna, J.R.Cort, P.K.Rajan, R.Xiao, R.Shastry, T.B.Acton, J.Liu, B.Rost, M.A.Kennedy, G.T.Montelione.

ABSTRACT

No abstract given.

Selected figure(s)

Figure 1.
Figure 1. (A) A subset of the multiple sequence alignment of the entire UPF0131 protein domain family (Pfam release 20.0) aligned using Clustal X.[4] Representatives from bacteria, archaea, and eukaryotes, indicated by their Swiss-Prot IDs, are listed in black, magenta, and green, respectively. Amino acid residues identical or similar in 67% of the entire family are shown in red and green, respectively; conserved residues were colored using the BOXSHADE server. The sequence numbering for yftP from E. coli and the secondary structural elements found in its NMR structure described in this paper (1XHS) are shown above the alignment. (B) Stereoview of the solution structure of ytfP showing the backbone atom superposition of the final ensemble of 10 conformers representing the solution structure of ytfP; -strand elements are shown in green, -helices are in red. (C) Stereoview of the ribbon representation of a representative conformer (lowest CNS energy) from the ensemble generated using MOLMOL.[5] The secondary structural elements are labeled.

Figure 2.
Figure 2. (A) GRASP[29] electrostatic potential surfaces showing the face of ytfP containing the positively charged (blue) cavity (top) and the opposite face of the protein (bottom). (B) Two ConSurf[30] images of ytfP based on the multiple sequence alignment of the entire UPF0131 protein domain family. Residue coloring, reflecting the degree of residue conservation over the entire family, ranges from magenta (highly conserved) to cyan (variable). (C) Ribbon diagrams of the pairwise structural alignments of the solution structure of E. coli ytfP (1XHS; residues 1-113), crystal structure of P. horikoshii Y828_PYRHO (1V306; 7-116 plus C-terminal tag), and crystal structure of M.

The above figures are reprinted by permission from John Wiley & Sons, Inc.: Proteins (2007, 68, 789-795) copyright 2007.

Figures were selected by the author.