PDBsum entry 1xhl

Oxidoreductase

PDB id: 1xhl

Contents

Protein chains

274 a.a. *

Ligands

NDP ×2

TNE ×2

Waters ×234

* Residue conservation analysis

PDB id:

1xhl

Name:

Oxidoreductase

Title:

Crystal structure of putative tropinone reductase-ii from caenorhabditis elegans with cofactor and substrate

Structure:

Short-chain dehydrogenase/reductase family member (5l265), putative tropinone reductase-ii. Chain: a, b. Engineered: yes

Source:

Caenorhabditis elegans. Organism_taxid: 6239. Gene: f25d1.5. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Biol. unit:

Tetramer (from PDB file)

Resolution:

2.40Å R-factor: 0.191 R-free: 0.215

Authors:

N.Schormann,E.Karpova,J.Zhou,Y.Zhang,J.Symersky,R.Bunzel,W.-Y.Huang, A.Arabshahi,S.Qiu,C.-H.Luan,R.Gray,M.Carson,J.Tsao,M.Luo,D.Johnson, S.Lu,G.Lin,D.Luo,Z.Cao,S.Li,A.Mckinstry,Q.Shang,Y.-J.Chen,T.Bray, L.Nagy,L.Delucas,Southeast Collaboratory For Structural Genomics (Secsg)

Key ref:

N.Schormann et al. Crystal structure of putative tropinone reductase-Ii from caenorhabditis elegans with cofactor and substrate. To be published, .

Date:

20-Sep-04 Release date: 28-Sep-04

Protein chains

Q19774  (Q19774_CAEEL) -  3-oxoacyl-[acyl-carrier-protein] reductase from Caenorhabditis elegans

Seq: 277 a.a.

Struc: 274 a.a.

Enzyme reactions

• Enzyme class: E.C.1.1.1.236 - tropinone reductase Ii.
• Reaction: pseudotropine + NADP⁺ = tropinone + NADPH + H⁺

pseudotropine (Bound ligand (Het Group name = NDP) corresponds exactly) + NADP(+) (Bound ligand (Het Group name = TNE) corresponds exactly) = tropinone + NADPH + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site