PDBsum entry 1vk5

Structural genomics, unknown function

PDB id: 1vk5

Contents

Protein chain

121 a.a.

Ligands

SO4 ×3

CPS

EDO ×4

Waters ×172

PDB id:

1vk5

Name:

Structural genomics, unknown function

Title:

X-ray structure of gene product from arabidopsis thaliana at3g22680

Structure:

Expressed protein. Chain: a. Engineered: yes

Source:

Arabidopsis thaliana. Thale cress. Organism_taxid: 3702. Gene: at3g22680. Expressed in: escherichia coli. Expression_system_taxid: 562

Biol. unit:

Dimer (from PQS)

Resolution:

1.60Å R-factor: 0.161 R-free: 0.184

Authors:

G.E.Wesenberg,D.W.Smith,G.N.Phillips Jr.,K.A.Johnson,C.A.Bingman, S.T.M.Allard,Center For Eukaryotic Structural Genomics (Cesg)

Key ref:

S.T.Allard et al. (2005). Structure at 1.6 A resolution of the protein from gene locus At3g22680 from Arabidopsis thaliana. Acta Crystallogr Sect F Struct Biol Cryst Commun, 61, 647-650. PubMed id: 16511118 DOI: 10.1107/S1744309105019743

Date:

06-May-04 Release date: 18-May-04

Protein chain

Q9LUJ3  (RDM1_ARATH) -  Protein RDM1 from Arabidopsis thaliana

Seq: 163 a.a.

Struc: 121 a.a.

DOI no: 10.1107/S1744309105019743

Acta Crystallogr Sect F Struct Biol Cryst Commun 61:647-650 (2005)

PubMed id: 16511118

Structure at 1.6 A resolution of the protein from gene locus At3g22680 from Arabidopsis thaliana.

S.T.Allard, C.A.Bingman, K.A.Johnson, G.E.Wesenberg, E.Bitto, W.B.Jeon, G.N.Phillips.

ABSTRACT

The gene product of At3g22680 from Arabidopsis thaliana codes for a protein of unknown function. The crystal structure of the At3g22680 gene product was determined by multiple-wavelength anomalous diffraction and refined to an R factor of 16.0% (Rfree = 18.4%) at 1.60 A resolution. The refined structure shows one monomer in the asymmetric unit, with one molecule of the non-denaturing detergent CHAPS {3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate} tightly bound. Protein At3g22680 shows no structural homology to any other known proteins and represents a new fold in protein conformation space.

Selected figure(s)

Figure 1.
A ribbon diagram of the crystal structure of At3g22680 color coded from the amino-terminus (blue) to the carboxy-terminus (red). CHAPS is depicted in yellow stick format with the elements color coded as follows: oxygen, red; nitrogen, blue; carbon, yellow. The figure was generated using PyMol (DeLano, 2002[triangle]). Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 July 1; 61(Pt 7): 647–650. Published online 2005 June 30. doi: 10.1107/S1744309105019743. Copyright [copyright] International Union of Crystallography 2005

Figure 2.
A ribbon representation of the At3g22680 homodimer. The two monomers are related by a crystallographic twofold axis and are shown in different colors (red and blue). The bound CHAPS is shown in the same format as in Fig. 1 [triangle] Figure 1-. The figure was generated using PyMol (DeLano, 2002[triangle]). Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 July 1; 61(Pt 7): 647–650. Published online 2005 June 30. doi: 10.1107/S1744309105019743. Copyright [copyright] International Union of Crystallography 2005

The above figures are reprinted from an Open Access publication published by the IUCr: Acta Crystallogr Sect F Struct Biol Cryst Commun (2005, 61, 647-650) copyright 2005.