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PDBsum entry 1vge
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Immunoglobulin
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PDB id
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1vge
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Contents |
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* Residue conservation analysis
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J Biol Chem
271:12191-12198
(1996)
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PubMed id:
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Structural studies of human autoantibodies. Crystal structure of a thyroid peroxidase autoantibody Fab.
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S.Chacko,
E.A.Padlan,
S.Portolano,
S.M.McLachlan,
B.Rapoport.
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ABSTRACT
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The three-dimensional structure of the Fab of TR1.9, a high-affinity IgG1, kappa
human autoantibody to thyroid peroxidase, was determined crystallographically to
a resolution of 2.0 A. The combining site was found to be relatively flat, like
other antibodies to large proteins. Sequence differences from the most closely
related germline genes mainly occur at positions occupied by residues with
outward-pointing side chains. An increased deformability of the second and third
complementarity-determining regions of the heavy chain may result from the
replacement of two germline asparagines and the presence of several glycines,
and may allow "induced fit" in the binding to antigen. Four exposed charged
residues, resulting from the use of a particular D (diversity) and J (joining)
segments in the assembly of the heavy chain, may contribute to the high affinity
of antigen binding. The crystal structure of TR1.9 Fab is the first for a human
IgG high-affinity autoantibody.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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W.Farrugia,
A.M.Scott,
and
P.A.Ramsland
(2009).
A possible role for metallic ions in the carbohydrate cluster recognition displayed by a lewis y specific antibody.
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PLoS One,
4,
e7777.
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PDB code:
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E.Carredano,
H.Baumann,
A.Grönberg,
N.Norrman,
G.Glad,
J.Zou,
O.Ersoy,
E.Steensma,
and
A.Axén
(2004).
A novel and conserved pocket of human kappa-Fab fragments: design, synthesis, and verification of directed affinity ligands.
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Protein Sci,
13,
1476-1488.
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H.Haruyama,
S.Ito,
K.Miyadai,
T.Takahashi,
R.Kawaida,
T.Takayama,
H.Hanzawa,
T.Hata,
J.Yamaguchi,
H.Yoshida-Kato,
K.Ichikawa,
J.Ohsumi,
S.Yonehara,
and
N.Serizawa
(2002).
Humanization of the mouse anti-Fas antibody HFE7A and crystal structure of the humanized HFE7A Fab fragment.
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Biol Pharm Bull,
25,
1537-1545.
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PDB code:
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H.R.Galea,
F.Bridoux,
J.C.Aldigier,
F.Paraf,
D.Bordessoule,
G.Touchard,
and
M.Cogné
(2002).
Molecular study of an IgG1kappa cryoglobulin yielding organized microtubular deposits and glomerulonephritis in the course of chronic lymphocytic leukaemia.
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Clin Exp Immunol,
129,
113-118.
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P.A.Ramsland,
and
W.Farrugia
(2002).
Crystal structures of human antibodies: a detailed and unfinished tapestry of immunoglobulin gene products.
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J Mol Recognit,
15,
248-259.
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B.Rapoport,
and
S.M.McLachlan
(2001).
Thyroid autoimmunity.
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J Clin Invest,
108,
1253-1259.
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L.Choulier,
V.Lafont,
N.Hugo,
and
D.Altschuh
(2000).
Covariance analysis of protein families: the case of the variable domains of antibodies.
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Proteins,
41,
475-484.
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S.M.McLachlan,
and
B.Rapoport
(2000).
Autoimmune response to the thyroid in humans: thyroid peroxidase--the common autoantigenic denominator.
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Int Rev Immunol,
19,
587-618.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
