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PDBsum entry 1vb5
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* Residue conservation analysis
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DOI no:
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Biochem Biophys Res Commun
319:725-732
(2004)
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PubMed id:
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Crystal structure of the regulatory subunit of archaeal initiation factor 2B (aIF2B) from hyperthermophilic archaeon Pyrococcus horikoshii OT3: a proposed structure of the regulatory subcomplex of eukaryotic IF2B.
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Y.Kakuta,
M.Tahara,
S.Maetani,
M.Yao,
I.Tanaka,
M.Kimura.
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ABSTRACT
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Eukaryotic translation initiation factor 2B (eIF2B) is the guanine-nucleotide
exchange factor for eukaryotic initiation factor 2 (eIF2). eIF2B is a
heteropentameric protein composed of alpha- subunits. The alpha, beta, and delta
subunits form a regulatory subcomplex, while the gamma and form a catalytic
subcomplex. Archaea possess homologues of alpha, beta, and delta subunits of
eIF2B. Here, we report the three-dimensional structure of an archaeal regulatory
subunit (aIF2Balpha) from the hyperthermophilic archaeon Pyrococcus horikoshii
OT3 determined by X-ray crystallography at 2.2A resolution. aIF2Balpha consists
of two subdomains, an N-domain (residues 1-95) and a C-domain (residues 96-276),
connected by a long alpha-helix (alpha5: 78-106). The N-domain contains a five
helix bundle structure, while the C-domain folds into the alpha/beta structure,
thus showing similarity to D-ribose-5-phosphate isomerase structure. The
presence of two molecules in the crystallographic asymmetric unit and the gel
filtration analysis suggest a dimeric structure of aIF2Balpha in solution,
interacting with each other by C-domains. Furthermore, the crystallographic
3-fold symmetry generates a homohexameric structure of aIF2Balpha; the
interaction is primarily mediated by the long alpha-helix at the N-domains. This
structure suggests an architecture of the three subunits, alpha, beta, and
delta, in the regulatory subcomplex within eIF2B.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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K.Dev,
T.J.Santangelo,
S.Rothenburg,
D.Neculai,
M.Dey,
F.Sicheri,
T.E.Dever,
J.N.Reeve,
and
A.G.Hinnebusch
(2009).
Archaeal aIF2B interacts with eukaryotic translation initiation factors eIF2alpha and eIF2Balpha: Implications for aIF2B function and eIF2B regulation.
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J Mol Biol,
392,
701-722.
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H.Tamura,
Y.Saito,
H.Ashida,
T.Inoue,
Y.Kai,
A.Yokota,
and
H.Matsumura
(2008).
Crystal structure of 5-methylthioribose 1-phosphate isomerase product complex from Bacillus subtilis: implications for catalytic mechanism.
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Protein Sci,
17,
126-135.
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PDB codes:
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A.G.Hinnebusch
(2005).
Translational regulation of GCN4 and the general amino acid control of yeast.
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Annu Rev Microbiol,
59,
407-450.
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H.Takagi,
Y.Kakuta,
T.Okada,
M.Yao,
I.Tanaka,
and
M.Kimura
(2005).
Crystal structure of archaeal toxin-antitoxin RelE-RelB complex with implications for toxin activity and antitoxin effects.
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Nat Struct Mol Biol,
12,
327-331.
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PDB code:
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H.Tamura,
H.Matsumura,
T.Inoue,
H.Ashida,
Y.Saito,
A.Yokota,
and
Y.Kai
(2005).
Crystallization and preliminary X-ray analysis of methylthioribose-1-phosphate isomerase from Bacillus subtilis.
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Acta Crystallogr Sect F Struct Biol Cryst Commun,
61,
595-598.
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P.Londei
(2005).
Evolution of translational initiation: new insights from the archaea.
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FEMS Microbiol Rev,
29,
185-200.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
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Links
