PDBsum entry 1v94

Oxidoreductase

PDB id: 1v94

Contents

Protein chains

429 a.a. *

Waters ×243

* Residue conservation analysis

PDB id:

1v94

Name:

Oxidoreductase

Title:

Crystal structure of isocitrate dehydrogenase from aeropyrum pernix

Structure:

Isocitrate dehydrogenase. Chain: a, b. Engineered: yes

Source:

Aeropyrum pernix. Organism_taxid: 56636. Gene: ape0689. Expressed in: escherichia coli. Expression_system_taxid: 562.

Biol. unit:

Dimer (from PQS)

Resolution:

2.28Å R-factor: 0.215 R-free: 0.256

Authors:

J.-J.Jeong,T.Sonoda,S.Fushinobu,H.Shoun,T.Wakagi

Key ref:

J.J.Jeong et al. (2004). Crystal structure of isocitrate dehydrogenase from Aeropyrum pernix. Proteins, 55, 1087-1089. PubMed id: 15146507 DOI: 10.1002/prot.20121

Date:

20-Jan-04 Release date: 25-Jan-05

Protein chains

Q9YE81  (Q9YE81_AERPE) -  isocitrate dehydrogenase (NADP(+)) from Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)

Seq: 432 a.a.

Struc: 429 a.a.

Enzyme reactions

• Enzyme class: E.C.1.1.1.42 - isocitrate dehydrogenase (NADP(+)).
• Pathway: Citric acid cycle
• Reaction: D-threo-isocitrate + NADP⁺ = 2-oxoglutarate + CO2 + NADPH
• Cofactor: Mn(2+) or Mg(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1002/prot.20121

Proteins 55:1087-1089 (2004)

PubMed id: 15146507

Crystal structure of isocitrate dehydrogenase from Aeropyrum pernix.

J.J.Jeong, T.Sonoda, S.Fushinobu, H.Shoun, T.Wakagi.

ABSTRACT

No abstract given.

Selected figure(s)

Figure 1.
Figure 1. Overall view of ApIDH dimer. Chains A and B are colored red and green, respectively. The three domains are indicated. The cysteine residues that form the disulfide bonds are shown as a ball-and-stick model.

Figure 2.
Figure 2. Comparison of the monomer structures of (a) ApIDH, (b) BsIDH and (c) EcIDH. Helices and strands are colored red and yellow respectively. The serine residue targeted for phophorylation of EcIDH (Ser113) and the corresponding residues of other IDHs are represented by black spheres.

The above figures are reprinted by permission from John Wiley & Sons, Inc.: Proteins (2004, 55, 1087-1089) copyright 2004.

Figures were selected by an automated process.