PDBsum entry 1v93

Oxidoreductase

PDB id

1v93

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Contents

			Protein chain

					292 a.a. *

			Ligands

					FAD


					DIO ×6

			Waters ×230

* Residue conservation analysis

PDB id:

1v93

Links

Name:

Oxidoreductase

Title:

5,10-methylenetetrahydrofolate reductase from thermus thermophilus hb8

Structure:

5,10-methylenetetrahydrofolate reductase. Chain: a. Engineered: yes

Source:

Thermus thermophilus. Organism_taxid: 300852. Strain: hb8. Expressed in: escherichia coli. Expression_system_taxid: 562.

Biol. unit:

Dimer (from PQS)

Resolution:

1.90Å

R-factor:

0.199

R-free:

0.236

Authors:

Y.Nakajima,I.Miyahara,K.Hirotsu,Riken Structural Genomics/proteomics Initiative (Rsgi)

Key ref:

Y.Nakajima et al. Crystal structure of 5,10-Methylenetetrahydrofolate reductase from thermus thermophilus hb8. To be published, .

Date:

20-Jan-04

Release date:

03-Feb-04

PROCHECK

	Headers

	References

Protein chain

Q9RA47 (Q9RA47_THETH) - Methylenetetrahydrofolate reductase (Fragment) from Thermus thermophilus

Seq: Struc:					229 a.a. 292 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 11 residue positions (black crosses)



		Enzyme reactions

Enzyme class:

E.C.1.5.1.20 - methylenetetrahydrofolate reductase [NAD(P)H].

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Pathway:

Folate Coenzymes

Reaction:

1.	(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NADP⁺ = (6R)-5,10- methylene-5,6,7,8-tetrahydrofolate + NADPH + H⁺
2.	(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD⁺ = (6R)-5,10- methylene-5,6,7,8-tetrahydrofolate + NADH + H⁺

(6S)-5-methyl-5,6,7,8-tetrahydrofolate	+	NADP(+)	=	(6R)-5,10- methylene-5,6,7,8-tetrahydrofolate	+	NADPH	+	H(+)

(6S)-5-methyl-5,6,7,8-tetrahydrofolate	+	NAD(+)	=	(6R)-5,10- methylene-5,6,7,8-tetrahydrofolate	+	NADH	+	H(+)

Cofactor:

FAD

FAD
Bound ligand (Het Group name = FAD) corresponds exactly

Molecule diagrams generated from .mol files obtained from the KEGG ftp site