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PDBsum entry 1v5b
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Oxidoreductase
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PDB id
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1v5b
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Contents |
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* Residue conservation analysis
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PDB id:
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| Name: |
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Oxidoreductase
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Title:
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The structure of the mutant, s225a and e251l, of 3-isopropylmalate dehydrogenase from bacillus coagulans
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Structure:
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3-isopropylmalate dehydrogenase. Chain: a, b, c, d, e, f, g, h. Engineered: yes. Mutation: yes
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Source:
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Bacillus coagulans. Organism_taxid: 1398. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Biol. unit:
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Dimer (from
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Resolution:
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2.95Å
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R-factor:
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0.214
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R-free:
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0.290
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Authors:
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K.Fujita,H.Minami,K.Suzuki,M.Tsunoda,T.Sekiguchi,R.Mizui,S.Tsuzaki, S.Nakamura,A.Takenaka
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Key ref:
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K.Fujita
et al.
Crystal structure of a highly thermo-Stabilized mutant of 3-Isopropylmalate dehydrogenase from bacillus coagulans: an evaluation of local packing density in the hydrophobic core.
To be published,
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Date:
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22-Nov-03
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Release date:
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15-Feb-05
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PROCHECK
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Headers
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References
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P12010
(LEU3_WEICA) -
3-isopropylmalate dehydrogenase from Weizmannia coagulans
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Seq:
Struc:
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366 a.a.
356 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 2 residue positions (black
crosses)
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Enzyme class:
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E.C.1.1.1.85
- 3-isopropylmalate dehydrogenase.
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Pathway:
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Leucine Biosynthesis
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Reaction:
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(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH
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(2R,3S)-3-isopropylmalate
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+
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NAD(+)
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=
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4-methyl-2-oxopentanoate
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+
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CO2
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+
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NADH
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Links
