PDBsum entry 1t8h

Structural genomics, unknown function

PDB id: 1t8h

Contents

Protein chain

272 a.a. *

Ligands

BME ×2

Metals

_ZN

Waters ×250

* Residue conservation analysis

PDB id:

1t8h

Name:

Structural genomics, unknown function

Title:

1.8 a crystal structure of an uncharacterized b. Stearothermophilus protein

Structure:

Ylmd protein sequence homologue. Chain: a. Engineered: yes

Source:

Geobacillus stearothermophilus. Organism_taxid: 1422. Gene: rbstp0554. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.80Å R-factor: 0.180 R-free: 0.210

Authors:

G.Minasov,L.Shuvalova,A.Mondragon,B.Taneja,S.F.Moy,F.R.Collart, W.F.Anderson,Midwest Center For Structural Genomics (Mcsg)

Key ref:

G.Minasov et al. 1.8 a crystal structure of an uncharacterized b. Stearothermophilus protein. To be published, .

Date:

12-May-04 Release date: 18-May-04

Protein chain

P84138  (PURNU_GEOS3) -  Purine nucleoside phosphorylase YlmD from Geobacillus stearothermophilus (strain DSM 13240 / CIP 106956 / 10)

Seq: 274 a.a.

Struc: 272 a.a.

Enzyme reactions

• Enzyme class 1: E.C.2.4.2.1 - purine-nucleoside phosphorylase.
• Reaction:
  1. a purine D-ribonucleoside + phosphate = a purine nucleobase + alpha- D-ribose 1-phosphate
  2. a purine 2'-deoxy-D-ribonucleoside + phosphate = a purine nucleobase + 2-deoxy-alpha-D-ribose 1-phosphate
• Enzyme class 2: E.C.2.4.2.28 - S-methyl-5'-thioadenosine phosphorylase.
• Reaction: S-methyl-5'-thioadenosine + phosphate = 5-(methylsulfanyl)-alpha-D-ribose 1-phosphate + adenine
• Enzyme class 3: E.C.3.5.4.4 - adenosine deaminase.
• Reaction:
  1. adenosine + H2O + H⁺ = inosine + NH4⁺
  2. 2'-deoxyadenosine + H2O + H⁺ = 2'-deoxyinosine + NH4⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site