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PDBsum entry 1spp

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protein Protein-protein interface(s) links
Complex (seminal plasma protein/spp) PDB id
1spp

 

 

 

 

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Contents
Protein chains
109 a.a. *
112 a.a. *
Waters ×237
* Residue conservation analysis
PDB id:
1spp
Name: Complex (seminal plasma protein/spp)
Title: The crystal structures of two members of the spermadhesin family reveal the folding of the cub domain
Structure: Major seminal plasma glycoprotein psp-i. Chain: a. Major seminal plasma glycoprotein psp-ii. Chain: b
Source: Sus scrofa. Pig. Organism_taxid: 9823. Organ: plasma. Tissue: sperm. Tissue: sperm
Biol. unit: Tetramer (from PQS)
Resolution:
2.40Å     R-factor:   0.200     R-free:   0.259
Authors: A.Romero,M.J.Romao,P.F.Varela,I.Kolln,J.M.Dias,A.L.Carvalho,L.Sanz, E.Topfer-Petersen,J.J.Calvete
Key ref: A.Romero et al. (1997). The crystal structures of two spermadhesins reveal the CUB domain fold. Nat Struct Biol, 4, 783-788. PubMed id: 9334740
Date:
19-Jun-97     Release date:   24-Jun-98    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P35495  (PSP1_PIG) -  Major seminal plasma glycoprotein PSP-I from Sus scrofa
Seq:
Struc:
133 a.a.
109 a.a.*
Protein chain
Pfam   ArchSchema ?
P35496  (PSP2_PIG) -  Major seminal plasma glycoprotein PSP-II from Sus scrofa
Seq:
Struc:
137 a.a.
112 a.a.
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 

 
Nat Struct Biol 4:783-788 (1997)
PubMed id: 9334740  
 
 
The crystal structures of two spermadhesins reveal the CUB domain fold.
A.Romero, M.J.Romão, P.F.Varela, I.Kölln, J.M.Dias, A.L.Carvalho, L.Sanz, E.Töpfer-Petersen, J.J.Calvete.
 
  ABSTRACT  
 
Spermadhesins, 12,000-14,000 M(r) mammalian proteins, include lectins involved in sperm-egg binding and display a single CUB domain architecture. We report the crystal structures of porcine seminal plasma PSP-I/PSP-II, a heterodimer of two glycosylated spermadhesins, and bovine aSFP at 2.4 A and 1.9 A resolution respectively.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20485747 D.Georgieva, K.Greunke, and C.Betzel (2010).
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  Mol Biosyst, 6, 1056-1060.  
20591323 M.L.Vadnais, and K.P.Roberts (2010).
Seminal plasma proteins inhibit in vitro- and cooling-induced capacitation in boar spermatozoa.
  Reprod Fertil Dev, 22, 893-900.  
19243221 D.Ng, G.M.Pitcher, R.K.Szilard, A.Sertié, M.Kanisek, S.J.Clapcote, T.Lipina, L.V.Kalia, D.Joo, C.McKerlie, M.Cortez, J.C.Roder, M.W.Salter, and R.R.McInnes (2009).
Neto1 is a novel CUB-domain NMDA receptor-interacting protein required for synaptic plasticity and learning.
  PLoS Biol, 7, e41.  
19658159 G.M.Wessel, and J.L.Wong (2009).
Cell surface changes in the egg at fertilization.
  Mol Reprod Dev, 76, 942-953.  
19473974 I.Bally, V.Rossi, T.Lunardi, N.M.Thielens, C.Gaboriaud, and G.J.Arlaud (2009).
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18303018 C.F.Tu, Y.T.Yan, S.Y.Wu, B.Djoko, M.T.Tsai, C.J.Cheng, and R.B.Yang (2008).
Domain and functional analysis of a novel platelet-endothelial cell surface protein, SCUBE1.
  J Biol Chem, 283, 12478-12488.  
18691891 D.C.Briggs, and A.J.Day (2008).
A bug in CUB's clothing: similarity between clostridial CBMs and complement CUBs.
  Trends Microbiol, 16, 407-408.  
18551302 E.I.Christensen, D.Raciti, L.Reggiani, P.J.Verroust, and A.W.Brändli (2008).
Gene expression analysis defines the proximal tubule as the compartment for endocytic receptor-mediated uptake in the Xenopus pronephric kidney.
  Pflugers Arch, 456, 1163-1176.  
17651403 E.M.García, J.M.Vázquez, I.Parrilla, M.D.Ortega, J.J.Calvete, L.Sanz, E.A.Martínez, J.Roca, and H.Rodríguez-Martínez (2008).
Localization and expression of spermadhesin PSP-I/PSP-II subunits in the reproductive organs of the boar.
  Int J Androl, 31, 408-417.  
18207079 L.K.Sirot, R.L.Poulson, M.C.McKenna, H.Girnary, M.F.Wolfner, and L.C.Harrington (2008).
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17538948 L.M.Melo, D.I.Teixeira, A.Havt, R.M.da Cunha, D.B.Martins, C.H.Castelletti, P.R.de Souza, J.L.Filho, V.J.Freitas, B.S.Cavada, and G.Rádis-Baptista (2008).
Buck (Capra hircus) genes encode new members of the spermadhesin family.
  Mol Reprod Dev, 75, 8.  
18020944 A.J.Ligtenberg, E.C.Veerman, A.V.Nieuw Amerongen, and J.Mollenhauer (2007).
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PDB codes: 2qqi 2qqj 2qqk 2qql 2qqm 2qqn 2qqo
17954916 F.S.Mathews, M.M.Gordon, Z.Chen, K.R.Rajashankar, S.E.Ealick, D.H.Alpers, and N.Sukumar (2007).
Crystal structure of human intrinsic factor: cobalamin complex at 2.6-A resolution.
  Proc Natl Acad Sci U S A, 104, 17311-17316.
PDB code: 2pmv
17446170 G.Blanc, B.Font, D.Eichenberger, C.Moreau, S.Ricard-Blum, D.J.Hulmes, and C.Moali (2007).
Insights into how CUB domains can exert specific functions while sharing a common fold: conserved and specific features of the CUB1 domain contribute to the molecular basis of procollagen C-proteinase enhancer-1 activity.
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17510169 I.Callebaut, J.P.Mornon, and P.Monget (2007).
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17005910 J.L.Wong, and G.M.Wessel (2006).
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  Mol Biol Cell, 17, 5241-5252.  
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Structure and function of secretory proteins of the male genital tract.
  Andrologia, 37, 202-204.  
15987500 G.C.Jones, and G.P.Riley (2005).
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15659374 I.Yanai, Y.Yu, X.Zhu, C.R.Cantor, and Z.Weng (2005).
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Analysis of the stability of the spermadhesin PSP-I/PSP-II heterodimer. Effects of Zn2+ and acidic pH.
  FEBS J, 272, 5663-5670.  
15888732 M.Ekhlasi-Hundrieser, K.Gohr, A.Wagner, M.Tsolova, A.Petrunkina, and E.Töpfer-Petersen (2005).
Spermadhesin AQN1 is a candidate receptor molecule involved in the formation of the oviductal sperm reservoir in the pig.
  Biol Reprod, 73, 536-545.  
15891391 S.K.Moestrup, and L.B.Nielsen (2005).
The role of the kidney in lipid metabolism.
  Curr Opin Lipidol, 16, 301-306.  
16141351 Z.Tao, Y.Peng, L.Nolasco, S.Cal, C.Lopez-Otin, R.Li, J.L.Moake, J.A.López, and J.F.Dong (2005).
Recombinant CUB-1 domain polypeptide inhibits the cleavage of ULVWF strings by ADAMTS13 under flow conditions.
  Blood, 106, 4139-4145.  
15345744 J.L.Mueller, D.R.Ripoll, C.F.Aquadro, and M.F.Wolfner (2004).
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PDB code: 1ouw
12724275 F.Centurion, J.M.Vazquez, J.J.Calvete, J.Roca, L.Sanz, I.Parrilla, E.M.Garcia, and E.A.Martinez (2003).
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X-ray structure of the Ca2+-binding interaction domain of C1s. Insights into the assembly of the C1 complex of complement.
  J Biol Chem, 278, 32157-32164.
PDB code: 1nzi
14561772 N.Kamei, and C.G.Glabe (2003).
The species-specific egg receptor for sea urchin sperm adhesion is EBR1,a novel ADAMTS protein.
  Genes Dev, 17, 2502-2507.  
12486138 S.Bernocco, B.M.Steiglitz, D.I.Svergun, M.V.Petoukhov, F.Ruggiero, S.Ricard-Blum, C.Ebel, C.Geourjon, G.Deleage, B.Font, D.Eichenberger, D.S.Greenspan, and D.J.Hulmes (2003).
Low resolution structure determination shows procollagen C-proteinase enhancer to be an elongated multidomain glycoprotein.
  J Biol Chem, 278, 7199-7205.  
12444055 A.M.Assreuy, J.J.Calvete, N.M.Alencar, B.S.Cavada, D.R.Rocha-Filho, S.C.Melo, F.Q.Cunha, and R.A.Ribeiro (2002).
Spermadhesin PSP-I/PSP-II heterodimer and its isolated subunits induced neutrophil migration into the peritoneal cavity of rats.
  Biol Reprod, 67, 1796-1803.  
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Characterization of neuropilin-1 structural features that confer binding to semaphorin 3A and vascular endothelial growth factor 165.
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Identification of the bacteria-binding peptide domain on salivary agglutinin (gp-340/DMBT1), a member of the scavenger receptor cysteine-rich superfamily.
  J Biol Chem, 277, 32109-32115.  
11854040 J.Dijkmans, J.Xu, S.Masure, S.Dhanaraj, A.Gosiewska, J.Geesin, J.Sprengel, S.Harris, P.Verhasselt, R.Gordon, and J.Yon (2002).
Characterization of platelet-derived growth factor-C (PDGF-C): expression in normal and tumor cells, biological activity and chromosomal localization.
  Int J Biochem Cell Biol, 34, 414-426.  
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Expression of spermadhesin genes in porcine male and female reproductive tracts.
  Mol Reprod Dev, 61, 32-41.  
11358517 L.Moschcovich, S.Bernocco, B.Font, H.Rivkin, D.Eichenberger, N.Chejanovsky, D.J.Hulmes, and E.Kessler (2001).
Folding and activity of recombinant human procollagen C-proteinase enhancer.
  Eur J Biochem, 268, 2991-2996.  
11195049 P.J.Verroust, and R.Kozyraki (2001).
The roles of cubilin and megalin, two multiligand receptors, in proximal tubule function: possible implication in the progression of renal disease.
  Curr Opin Nephrol Hypertens, 10, 33-38.  
11375443 S.K.Moestrup, and P.J.Verroust (2001).
Megalin- and cubilin-mediated endocytosis of protein-bound vitamins, lipids, and hormones in polarized epithelia.
  Annu Rev Nutr, 21, 407-428.  
10787174 S.K.Moestrup, and R.Kozyraki (2000).
Cubilin, a high-density lipoprotein receptor.
  Curr Opin Lipidol, 11, 133-140.  
10497325 E.Töpfer-Petersen (1999).
Molecules on the sperm's route to fertilization.
  J Exp Zool, 285, 259-266.  
10504403 M.Nimtz, E.Grabenhorst, H.S.Conradt, L.Sanz, and J.J.Calvete (1999).
Structural characterization of the oligosaccharide chains of native and crystallized boar seminal plasma spermadhesin PSP-I and PSP-II glycoforms.
  Eur J Biochem, 265, 703-718.  
9988213 P.M.Wassarman (1999).
Mammalian fertilization: molecular aspects of gamete adhesion, exocytosis, and fusion.
  Cell, 96, 175-183.  
10485905 U.Holmskov, J.Mollenhauer, J.Madsen, L.Vitved, J.Gronlund, I.Tornoe, A.Kliem, K.B.Reid, A.Poustka, and K.Skjodt (1999).
Cloning of gp-340, a putative opsonin receptor for lung surfactant protein D.
  Proc Natl Acad Sci U S A, 96, 10794-10799.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.

 

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