PDBsum entry 1rnt

Hydrolase(endoribonuclease)

PDB id: 1rnt

Contents

Protein chain

104 a.a. *

Ligands

2GP

Waters ×91

* Residue conservation analysis

PDB id:

1rnt

Name:

Hydrolase(endoribonuclease)

Title:

Restrained least-squares refinement of the crystal structure of the ribonuclease t1(asterisk)2(prime)-guanylic acid complex at 1.9 angstroms resolution

Structure:

Ribonuclease t1 isozyme. Chain: a. Engineered: yes

Source:

Aspergillus oryzae. Organism_taxid: 5062

Resolution:

1.90Å R-factor: 0.191

Authors:

W.Saenger,R.Arni,U.Heinemann,R.Tokuoka

Key ref:

R.Arni et al. (1987). Restrained least-Squares refinement of the crystal structure of the ribonuclease t1(asterisk)2(prime)-Guanylic acid complex at 1.9 angstroms resolution. Acta crystallogr ,Sect b, 43, 549.

Date:

10-Jul-87 Release date: 16-Oct-87

Protein chain

P00651  (RNT1_ASPOR) -  Guanyl-specific ribonuclease T1 from Aspergillus oryzae (strain ATCC 42149 / RIB 40)

Seq: 130 a.a.

Struc: 104 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.4.6.1.24 - ribonuclease T1.
• Reaction: [RNA] containing guanosine + H2O = an [RNA fragment]-3'-guanosine- 3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment]