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PDBsum entry 1qnq
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protein ligands links
Hydrolase PDB id
1qnq

 

 

 

 

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Contents
Protein chain
344 a.a. *
Ligands
TPT ×2
SO4 ×2
NAG ×4
Waters ×654
* Residue conservation analysis
PDB id:
1qnq
Name: Hydrolase
Title: The 3-d structure of a trichoderma reesei b-mannanase from glycoside hydrolase family 5
Structure: Endo-1,4-b-d-mannanase. Chain: a. Fragment: catalytic domain, residues 28-371. Engineered: yes
Source: Trichoderma reesei. Organism_taxid: 51453. Strain: alko4330. Expressed in: trichoderma reesei. Expression_system_taxid: 51453.
Resolution:
1.65Å     R-factor:   0.115     R-free:   0.160
Authors: E.Sabini,H.Schubert,G.Murshudov,K.S.Wilson,M.Siika-Aho,M.Penttila
Key ref:
E.Sabini et al. (2000). The three-dimensional structure of a Trichoderma reesei beta-mannanase from glycoside hydrolase family 5. Acta Crystallogr D Biol Crystallogr, 56, 3. PubMed id: 10666621 DOI: 10.1107/S0907444999013943
Date:
20-Oct-99     Release date:   19-Oct-00    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q99036  (MANA_HYPJR) -  Mannan endo-1,4-beta-mannosidase A from Hypocrea jecorina (strain ATCC 56765 / BCRC 32924 / NRRL 11460 / Rut C-30)
Seq:
Struc: 		437 a.a.
344 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.3.2.1.78  - mannan endo-1,4-beta-mannosidase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Random hydrolysis of 1,4-beta-D-mannosidic linkages in mannans, galactomannans, glucomannans, and galactoglucomannans.

 

 
DOI no: 10.1107/S0907444999013943 Acta Crystallogr D Biol Crystallogr 56:3 (2000)
PubMed id: 10666621  
 
 
The three-dimensional structure of a Trichoderma reesei beta-mannanase from glycoside hydrolase family 5.
E.Sabini, H.Schubert, G.Murshudov, K.S.Wilson, M.Siika-Aho, M.Penttilä.
 
  ABSTRACT  
 
The crystal structure of the catalytic core domain of beta-mannanase from the fungus Trichoderma reesei has been determined at a resolution of 1.5 A. The structure was solved using the anomalous scattering from a single non-isomorphous platinum complex with two heavy-metal sites in space group P2(1). The map computed with the experimental phases was enhanced by the application of an automated model building and refinement procedure using the amplitudes and experimental phases as observations. This approach is expected to be of more general application. The structure of the native enzyme and complexes with Tris-HCl and mannobiose are also reported: the mannobiose binds in subsites +1 and +2. The structure is briefly compared with that of the homologous beta-mannanase from the bacterium Thermomonospora fusca.
 
  Selected figure(s)  
 
Figure 1.
Figure 1 (a) Anomalous and (b) isomorphous difference Patterson syntheses at 1.65 Å resolution for the Pty complex: the Harker vectors of the two platinum sites are indicated. 		Figure 5.
Figure 5 As Fig. 4-. Aromatic residues (here Trp164 is shown) had a `hole' at the centre of the ring in the REFMAC/ARP map (c).
 
  The above figures are reprinted by permission from the IUCr: Acta Crystallogr D Biol Crystallogr (2000, 56, 3-0) copyright 2000.  
  Figures were selected by an automated process.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
21460844 B.P.Callahan, N.I.Topilina, M.J.Stanger, P.Van Roey, and M.Belfort (2011).
Structure of catalytically competent intein caught in a redox trap with functional and evolutionary implications.
  Nat Struct Mol Biol, 18, 630-633.
PDB code: 3nzm
19912637 B.C.Do, T.T.Dang, J.G.Berrin, D.Haltrich, K.A.To, J.C.Sigoillot, and M.Yamabhai (2009).
Cloning, expression in Pichia pastoris, and characterization of a thermostable GH5 mannan endo-1,4-beta-mannosidase from Aspergillus niger BK01.
  Microb Cell Fact, 8, 59.  
  18323611 Y.Zhang, F.Gao, Y.Xue, Y.Zeng, H.Peng, J.Qi, and Y.Ma (2008).
Crystallization and preliminary X-ray study of native and selenomethionyl beta-1,4-mannanase AaManA from Alicyclobacillus acidocaldariusTc-12-31.
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 64, 209-212.  
18755688 Y.Zhang, J.Ju, H.Peng, F.Gao, C.Zhou, Y.Zeng, Y.Xue, Y.Li, B.Henrissat, G.F.Gao, and Y.Ma (2008).
Biochemical and Structural Characterization of the Intracellular Mannanase AaManA of Alicyclobacillus acidocaldarius Reveals a Novel Glycoside Hydrolase Family Belonging to Clan GH-A.
  J Biol Chem, 283, 31551-31558.
PDB code: 3civ
17287210 L.E.Tailford, V.A.Money, N.L.Smith, C.Dumon, G.J.Davies, and H.J.Gilbert (2007).
Mannose foraging by Bacteroides thetaiotaomicron: structure and specificity of the beta-mannosidase, BtMan2A.
  J Biol Chem, 282, 11291-11299.
PDB code: 2je8
16240096 E.Papaleo, P.Fantucci, M.Vai, and L.De Gioia (2006).
Three-dimensional structure of the catalytic domain of the yeast beta-(1,3)-glucan transferase Gas1: a molecular modeling investigation.
  J Mol Model, 12, 237-248.  
15840830 R.Bourgault, A.J.Oakley, J.D.Bewley, and M.C.Wilce (2005).
Three-dimensional structure of (1,4)-beta-D-mannan mannanohydrolase from tomato fruit.
  Protein Sci, 14, 1233-1241.
PDB code: 1rh9
12364331 L.K.Skov, O.Mirza, D.Sprogøe, I.Dar, M.Remaud-Simeon, C.Albenne, P.Monsan, and M.Gajhede (2002).
Oligosaccharide and sucrose complexes of amylosucrase. Structural implications for the polymerase activity.
  J Biol Chem, 277, 47741-47747.
PDB codes: 1mvy 1mw0 1mw1 1mw2 1mw3
10759859 E.Sabini, K.S.Wilson, M.Siika-aho, C.Boisset, and H.Chanzy (2000).
Digestion of single crystals of mannan I by an endo-mannanase from Trichoderma reesei.
  Eur J Biochem, 267, 2340-2344.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

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