PDBsum entry 1pzc

Electron transfer (cuproprotein)

PDB id

1pzc

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Contents

			Protein chain

					122 a.a. *

			Waters ×137

* Residue conservation analysis

PDB id:

1pzc

Links
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Name:

Electron transfer (cuproprotein)

Title:

Apo-pseudoazurin (metal free protein)

Structure:

Pseudoazurin. Chain: a. Engineered: yes. Other_details: apo form

Source:

Alcaligenes faecalis. Organism_taxid: 511. Strain: s-6. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.85Å

R-factor:

0.164

R-free:

0.215

Authors:

K.Petratos

Key ref:

K.Petratos et al. (1995). The crystal structure of apo-pseudoazurin from Alcaligenes faecalis S-6. FEBS Lett, 368, 432-434. PubMed id: 7635192

Date:

22-Feb-95

Release date:

15-Sep-95

PROCHECK

	Headers

	References

Protein chain

P04377 (AZUP_ALCFA) - Pseudoazurin from Alcaligenes faecalis

Seq: Struc:					146 a.a. 122 a.a.

Key:

PfamA domain

Secondary structure

CATH domain

	FEBS Lett 368:432-434 (1995)
PubMed id: 7635192

The crystal structure of apo-pseudoazurin from Alcaligenes faecalis S-6.
K.Petratos, M.Papadovasilaki, Z.Dauter.

ABSTRACT

The 3D structure of the apo-pseudoazurin (copper free pseudoazurin) from Alcaligenes faecalis strain S-6 is determined and refined at pH 6.7 using X-ray diffraction data to 1.85 A resolution. The final crystallographic R-factor is 0.164. Comparing the structures of apo-pseudoazurin and the native (Cu2+) protein, we observed limited differences ranging between 0.1-0.4 A at the vicinity of the copper site, at the loops connecting the secondary structural elements, at certain beta-strands and at the amino and carboxy termini of the protein.

Literature references that cite this PDB file's key reference

PubMed id

Reference

20945335

R.Gessmann, C.Kyvelidou, M.Papadovasilaki, and K.Petratos (2011).
The crystal structure of cobalt-substituted pseudoazurin from Alcaligenes faecalis.

Biopolymers, 95, 202-207.

PDB code:

3nyk

16881076

A.Stirpe, L.Sportelli, and R.Guzzi (2006).
A comparative investigation of the thermal unfolding of pseudoazurin in the Cu(II)-holo and apo form.

Biopolymers, 83, 487-497.

15274913

J.Gough, and C.Chothia (2004).
The linked conservation of structure and function in a family of high diversity: the monomeric cupredoxins.

Structure, 12, 917-925.

11369860

J.S.Reader, N.A.Van Nuland, G.S.Thompson, S.J.Ferguson, C.M.Dobson, and S.E.Radford (2001).
A partially folded intermediate species of the beta-sheet protein apo-pseudoazurin is trapped during proline-limited folding.

Protein Sci, 10, 1216-1224.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.