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PDBsum entry 1pmc
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Proteinase inhibitor
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PDB id
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1pmc
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J Mol Biol
258:158-171
(1996)
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PubMed id:
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Solution structure of PMP-C: a new fold in the group of small serine proteinase inhibitors.
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G.Mer,
H.Hietter,
C.Kellenberger,
M.Renatus,
B.Luu,
J.F.Lefèvre.
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ABSTRACT
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The solution structure and the disulfide pairings of a 36-residue proteinase
inhibitor isolated from the insect Locusta migratoria have been determined using
NMR spectroscopy and simulated annealing calculations. The peptide, termed
PMP-C, was previously shown to inhibit bovine alpha-chymotrypsin as well as
human leukocyte elastase, and was also found to block high-voltage-activated
Ca2+ currents in rat sensory neurones. PMP-C has a prolate ellipsoid shape and
adopts a tertiary fold hitherto unobserved in the large group of small
"canonical" proteinase inhibitors. The over-all fold consists mainly of three
strands arranged in a right-handed twisted, antiparallel, beta-sheet that
demarcates a cavity, together with a linear amino-terminal segment oriented
almost perpendicular to the three strands of the beta-sheet. Inside the cavity a
phenyl ring constitutes the centre of a hydrophobic core. The proteinase binding
loop is located in the carboxy-terminal part of the molecule, between two
cysteine residues involved in disulfide bridges. Its conformation resembles that
found in other small canonical proteinase inhibitors. A comparison of PMP-C
structure with the recently published solution structure of the related peptide
PMP-D2 shows that the most significant differences are complementary changes
involved in the stabilization of similar folds. This comparison led us to review
the structure of PMP-D2 and to identify two salt bridges in PMP-D2.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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B.Breugelmans,
G.Simonet,
V.van Hoef,
S.Van Soest,
and
J.Vanden Broeck
(2009).
Identification, distribution and molecular evolution of the pacifastin gene family in Metazoa.
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BMC Evol Biol,
9,
97.
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B.Breugelmans,
G.Simonet,
V.van Hoef,
I.Claeys,
S.Van Soest,
and
J.Vanden Broeck
(2008).
Quantitative RT-PCR analysis of pacifastin-related precursor transcripts during the reproductive cycle of solitarious and gregarious desert locusts.
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Insect Mol Biol,
17,
137-145.
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P.Leone,
A.Roussel,
and
C.Kellenberger
(2008).
Structure of Locusta migratoria protease inhibitor 3 (LMPI-3) in complex with Fusarium oxysporum trypsin.
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Acta Crystallogr D Biol Crystallogr,
64,
1165-1171.
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PDB code:
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S.Roy,
P.Aravind,
C.Madhurantakam,
A.K.Ghosh,
R.Sankaranarayanan,
and
A.K.Das
(2006).
Crystallization and preliminary X-ray diffraction analysis of a protease inhibitor from the haemolymph of the Indian tasar silkworm Antheraea mylitta.
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Acta Crystallogr Sect F Struct Biol Cryst Commun,
62,
669-671.
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Z.Gáspári,
B.Szenthe,
A.Patthy,
W.M.Westler,
L.Gráf,
and
A.Perczel
(2006).
Local binding with globally distributed changes in a small protease inhibitor upon enzyme binding.
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FEBS J,
273,
1831-1842.
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S.F.Foley,
H.W.van Vlijmen,
R.E.Boynton,
H.B.Adkins,
A.E.Cheung,
J.Singh,
M.Sanicola,
C.N.Young,
and
D.Wen
(2003).
The CRIPTO/FRL-1/CRYPTIC (CFC) domain of human Cripto. Functional and structural insights through disulfide structure analysis.
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Eur J Biochem,
270,
3610-3618.
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H.Hegyi,
J.Lin,
D.Greenbaum,
and
M.Gerstein
(2002).
Structural genomics analysis: characteristics of atypical, common, and horizontally transferred folds.
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Proteins,
47,
126-141.
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V.M.Panin,
L.Shao,
L.Lei,
D.J.Moloney,
K.D.Irvine,
and
R.S.Haltiwanger
(2002).
Notch ligands are substrates for protein O-fucosyltransferase-1 and Fringe.
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J Biol Chem,
277,
29945-29952.
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Z.Gáspári,
A.Patthy,
L.Gráf,
and
A.Perczel
(2002).
Comparative structure analysis of proteinase inhibitors from the desert locust, Schistocerca gregaria.
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Eur J Biochem,
269,
527-537.
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PDB codes:
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A.Roussel,
M.Mathieu,
A.Dobbs,
B.Luu,
C.Cambillau,
and
C.Kellenberger
(2001).
Complexation of two proteic insect inhibitors to the active site of chymotrypsin suggests decoupled roles for binding and selectivity.
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J Biol Chem,
276,
38893-38898.
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PDB codes:
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Z.Malik,
S.Amir,
G.Pál,
Z.Buzás,
E.Várallyay,
J.Antal,
Z.Szilágyi,
K.Vékey,
B.Asbóth,
A.Patthy,
and
L.Gráf
(1999).
Proteinase inhibitors from desert locust, Schistocerca gregaria: engineering of both P(1) and P(1)' residues converts a potent chymotrypsin inhibitor to a potent trypsin inhibitor.
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Biochim Biophys Acta,
1434,
143-150.
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P.Holliger,
and
L.Riechmann
(1997).
A conserved infection pathway for filamentous bacteriophages is suggested by the structure of the membrane penetration domain of the minor coat protein g3p from phage fd.
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Structure,
5,
265-275.
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PDB code:
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R.H.Scott,
V.J.Gorton,
L.Harding,
D.Patel,
S.Pacey,
C.Kellenberger,
H.Hietter,
and
I.Bermudez
(1997).
Inhibition of neuronal high voltage-activated calcium channels by insect peptides: a comparison with the actions of omega-conotoxin GVIA.
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Neuropharmacology,
36,
195-208.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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