PDBsum entry 1oho

Isomerase

PDB id: 1oho

Contents

Protein chain

123 a.a. *

Ligands

EQU

Waters ×42

* Residue conservation analysis

PDB id:

1oho

Name:

Isomerase

Title:

Crystal structure of ketosteroid isomerase y16f/d40n mutant complexed with equilenin

Structure:

Steroid delta-isomerase. Chain: a. Synonym: delta-5-3-ketosteroid, isomerase. Engineered: yes. Mutation: yes

Source:

Pseudomonas putida. Organism_taxid: 303. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Biol. unit:

Dimer (from PDB file)

Resolution:

1.90Å R-factor: 0.196 R-free: 0.226

Authors:

M.-S.Kim,M.Byun,B.-H.Oh

Key ref:

M.-S.Kim et al. Crystal structure of ketosteroid isomerase y16f/d40n mutant complexed with equilenin. To be published, .

Date:

29-May-03 Release date: 31-Mar-05

Protein chain

P07445  (SDIS_PSEPU) -  Steroid Delta-isomerase from Pseudomonas putida

Seq: 131 a.a.

Struc: 123 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.5.3.3.1 - steroid Delta-isomerase.
• Reaction: a 3-oxo-Delta₅-steroid = a 3-oxo-Delta₄-steroid

3-oxo-Delta(5)-steroid (Bound ligand (Het Group name = EQU) matches with 90.48% similarity) = 3-oxo-Delta(4)-steroid

Molecule diagrams generated from .mol files obtained from the KEGG ftp site