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PDBsum entry 1od8
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protein ligands metals links
Hydrolase/lectin PDB id
1od8

 

 

 

 

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Contents
Protein chain
302 a.a. *
Ligands
XDL-XYP ×2
IMD ×2
Metals
_NA ×4
Waters ×629
* Residue conservation analysis
PDB id:
1od8
Name: Hydrolase/lectin
Title: Xylanase xyn10a from streptomyces lividans in complex with xylobio- isofagomine lactam
Structure: Endo-1,4-beta-xylanase a. Chain: a. Fragment: catalytic module, residues 42-354. Synonym: xylanase a. Engineered: yes
Source: Streptomyces lividans. Organism_taxid: 1916. Expressed in: streptomyces lividans. Expression_system_taxid: 1916.
Resolution:
1.05Å     R-factor:   0.104     R-free:   0.126
Authors: T.M.Gloster,S.Roberts,G.J.Davies
Key ref: T.Gloster et al. (2003). A xylobiose-derived isofagomine lactam glycosidase inhibitor binds as its amide tautomer. Chem Commun (camb), 8, 944-945. PubMed id: 12744311
Date:
14-Feb-03     Release date:   08-Apr-03    
PROCHECK
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 Headers
 References

Protein chain
P26514  (XYNA_STRLI) -  Endo-1,4-beta-xylanase A from Streptomyces lividans
Seq:
Struc: 		477 a.a.
302 a.a.
Key:    Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.3.2.1.8  - endo-1,4-beta-xylanase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Endohydrolysis of 1,4-beta-D-xylosidic linkages in xylans.

 

 
Chem Commun (camb) 8:944-945 (2003)
PubMed id: 12744311  
 
 
A xylobiose-derived isofagomine lactam glycosidase inhibitor binds as its amide tautomer.
T.Gloster, S.J.Williams, C.A.Tarling, S.Roberts, C.Dupont, P.Jodoin, F.Shareck, S.G.Withers, G.J.Davies.
 
  ABSTRACT  
 
The atomic-resolution structure of a xylobiose-derived isofagomine lactam in complex with the xylanase Xyn10A from Streptomyces lividans reveals that the lactam is bound to the enzyme as the amide tautomer, with "reversed" protonation-states for nucleophile and acid-base.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
18074341 A.D.Hill, and P.J.Reilly (2008).
A Gibbs free energy correlation for automated docking of carbohydrates.
  J Comput Chem, 29, 1131-1141.  
15515081 F.Vincent, T.M.Gloster, J.Macdonald, C.Morland, R.V.Stick, F.M.Dias, J.A.Prates, C.M.Fontes, H.J.Gilbert, and G.J.Davies (2004).
Common inhibition of both beta-glucosidases and beta-mannosidases by isofagomine lactam reflects different conformational itineraries for pyranoside hydrolysis.
  Chembiochem, 5, 1596-1599.
PDB codes: 1uz1 1uz4
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.

 

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