PDBsum entry 1ocp

DNA binding protein

PDB id: 1ocp

Contents

Protein chain

67 a.a. *

* Residue conservation analysis

PDB id:

1ocp

Name:

DNA binding protein

Title:

Solution structure of oct3 pou-homeodomain

Structure:

Oct-3. Chain: a. Engineered: yes

Source:

Mus musculus. House mouse. Organism_taxid: 10090. Gene: t7 gene 1 under control of the. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Promoter

NMR struc:

20 models

Authors:

E.H.Morita,F.Hayashi,M.Shirakawa,Y.Kyogoku

Key ref:

E.H.Morita et al. (1995). Structure of the Oct-3 POU-homeodomain in solution, as determined by triple resonance heteronuclear multidimensional NMR spectroscopy. Protein Sci, 4, 729-739. PubMed id: 7613470 DOI: 10.1002/pro.5560040412

Date:

21-Feb-95 Release date: 15-Sep-95

Protein chain

P20263  (PO5F1_MOUSE) -  POU domain, class 5, transcription factor 1 from Mus musculus

Seq: 352 a.a.

Struc: 67 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.?

DOI no: 10.1002/pro.5560040412

Protein Sci 4:729-739 (1995)

PubMed id: 7613470

Structure of the Oct-3 POU-homeodomain in solution, as determined by triple resonance heteronuclear multidimensional NMR spectroscopy.

E.H.Morita, M.Shirakawa, F.Hayashi, M.Imagawa, Y.Kyogoku.

ABSTRACT

The POU-homeodomain (POUH) forms the bipartite DNA-binding POU domain in association with the POU-specific domain. The 1H, 15N, and 13C magnetic resonances of the 67-amino acid long POUH of mouse Oct-3 have almost completely been assigned, mainly through the combined use of three-dimensional triple resonance NMR methods. Based on the distance and dihedral angle constraints derived from the NMR data, the solution structure of the POUH domain has been calculated by the ab initio simulated annealing method. The average RMS deviation for all backbone heavy atoms of the 20 best calculated structures for residues 9-53 of the total 67 amino acid residues is 0.44 A. The POUH domain consists of three alpha-helices (helix-I, 10-20; helix-II, 28-38; and helix-III, 42-53), and helices-II and -III form a helix-turn-helix motif. In comparison with other classical homeodomains, the folding of the three helices is quite similar. However, the length of helix-III is fairly short. In the complex of the Oct-1 POU domain with an octamer site (Klemm JD, et al., 1994, Cell 77:21-32), the corresponding region is involved in helix-III. The structural difference between these two cases will be discussed.