PDBsum entry 1lqn

Protein binding

PDB id: 1lqn

Contents

Protein chain

207 a.a.

Theoretical model

PDB id:

1lqn

Name:

Protein binding

Title:

Theoritical model of human metalloproteinase inhibitor 1

Structure:

Metalloproteinase inhibitor 1. Chain: a. Synonym: timp-1

Source:

Homo sapiens. Human

Authors:

S.C.Mallena,K.Yadugiri

Key ref:

K.Saikrishnan et al. (2002). Domain closure and action of uracil DNA glycosylase (UDG): structures of new crystal forms containing the Escherichia coli enzyme and a comparative study of the known structures involving UDG. Acta Crystallogr D Biol Crystallogr, 58, 1269-1276. PubMed id: 12136137 DOI: 10.1107/S0907444902009599

Date:

11-May-02 Release date: 05-Jun-02

Protein chain

P01033  (TIMP1_HUMAN) -  Metalloproteinase inhibitor 1

Seq: 207 a.a.

Struc: 207 a.a.

DOI no: 10.1107/S0907444902009599

Acta Crystallogr D Biol Crystallogr 58:1269-1276 (2002)

PubMed id: 12136137

Domain closure and action of uracil DNA glycosylase (UDG): structures of new crystal forms containing the Escherichia coli enzyme and a comparative study of the known structures involving UDG.

K.Saikrishnan, M.Bidya Sagar, R.Ravishankar, S.Roy, K.Purnapatre, P.Handa, U.Varshney, M.Vijayan.

ABSTRACT

The structures of a new crystal form of free Escherichia coli uracil DNA glycosylase (UDG), containing four molecules in the asymmetric unit, and two forms of its complex with the proteinaceous inhibitor Ugi, containing two and four crystallographically independent complexes, have been determined. A comparison of these structures and the already known crystal structures containing UDG shows that the enzyme can be considered to be made up of two independently moving structural entities or domains. A detailed study of free and DNA-bound human enzyme strengthens this conclusion. The domains close upon binding to uracil-containing DNA, whereas they do not appear to do so upon binding to Ugi. The comparative study also shows that the mobility of the molecule involves the rigid-body movement of the domains superposed on flexibility within domains.

Selected figure(s)

Figure 3.
Figure 3 Delineation of domains in EcUDG. Domain 1 is in green and domain 2 is in red. The linker region is in purple. The axis about which the domains move during closure is also shown.

Figure 4.
Figure 4 Stereoview illustrating domain closure in EcUDG. Domain 1 of the free enzyme (dark) and that of the enzyme complexed with single-stranded DNA (light) are superposed. The axis about which the molecule has to rotate to bring domain 2 into superposition is perpendicular to the figure and is indicated by a ball.

The above figures are reprinted by permission from the IUCr: Acta Crystallogr D Biol Crystallogr (2002, 58, 1269-1276) copyright 2002.

Figures were selected by an automated process.