|
|
|
|
|
 |
Contents |
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
* Residue conservation analysis
|
|
|
|
|
|
PDB id:
|
|
|
|
| Name: |
|
Lectin
|
|
|
Title:
|
|
X-ray structure of a (alpha-man(1-3)beta-man(1-4)glcnac)-lectin complex at 2.1 angstroms resolution
|
|
Structure:
|
|
Legume isolectin i (alpha chain). Chain: a, c. Legume isolectin i (beta chain). Chain: b, d
|
|
Source:
|
|
Lathyrus ochrus. Yellow-flowered pea. Organism_taxid: 3858. Organ: seed. Organ: seed
|
|
Biol. unit:
|
|
Tetramer (from
)
|
|
Resolution:
|
|
|
|
Authors:
|
|
Y.Bourne,C.Cambillau
|
|
Key ref:
|
|
Y.Bourne
et al.
(1990).
X-ray structure of a (alpha-Man(1-3)beta-Man(1-4)GlcNAc)-lectin complex at 2.1-A resolution. The role of water in sugar-lectin interaction.
J Biol Chem,
265,
18161-18165.
PubMed id:
|
|
|
Date:
|
|
|
27-Jan-94
|
Release date:
|
30-Apr-94
|
|
|
|
|
|
|
PROCHECK
|
|
|
|
|
|
Headers
|
|
|
|
References
|
|
|
|
|
|
|
|
P04122
(LECB_LATOC) -
Lectin beta-1 and beta-2 chains from Lathyrus ochrus
|
|
|
|
Seq:
Struc:
|
|
|
|
181 a.a.
180 a.a.*
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Enzyme class:
|
|
Chains A, B, C, D:
E.C.?
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
| |
|
|
J Biol Chem
265:18161-18165
(1990)
|
|
PubMed id:
|
|
|
|
|
|
| |
|
X-ray structure of a (alpha-Man(1-3)beta-Man(1-4)GlcNAc)-lectin complex at 2.1-A resolution. The role of water in sugar-lectin interaction.
|
|
Y.Bourne,
P.Rougé,
C.Cambillau.
|
|
|
|
|
| |
ABSTRACT
|
|
|
|
| |
|
|
We describe herein the high resolution refined x-ray structure of a
trisaccharide, which is a part of the N-acetyllactosamine type glycan found in
the majority of the N-glycosyl-proteins, complexed to the isolectin I. According
to the potentials used by Imberty et al. (Imburty, A., Gerber, S., Tran, V., and
Pérez, S. (1990) Glycoconjugate J. 7, 27-54) the trisaccharide is in a
low-energy state. Only one mannose moiety establishes direct hydrogen bonds with
the lectin, as it is the case for monosaccharide-lectin complexes. The
comparison of our trisaccharide with the one determined in solution by Warin et
al. (Warin, V., Baert, F., Fouret, R., Strecker, G., Fournet, B., and Montreuil,
J. (1979) Carbohydr. Res. 76, 11-22) shows that both adopt roughly the same
conformation. The differences in these two sugar structures allow us to assign
the role of water molecules present in the vicinity of our trisaccharide for the
stabilization of this sugar-lectin complex.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
|
Literature references that cite this PDB file's key reference
|
|
|
| |
PubMed id
|
|
Reference
|
|
|
|
|
|
Q.Vicens,
and
E.Westhof
(2003).
Molecular recognition of aminoglycoside antibiotics by ribosomal RNA and resistance enzymes: an analysis of x-ray crystal structures.
|
| |
Biopolymers,
70,
42-57.
|
|
|
|
|
|
|
J.C.Rosa,
P.S.De Oliveira,
R.Garratt,
L.Beltramini,
K.Resing,
M.C.Roque-Barreira,
and
L.J.Greene
(1999).
KM+, a mannose-binding lectin from Artocarpus integrifolia: amino acid sequence, predicted tertiary structure, carbohydrate recognition, and analysis of the beta-prism fold.
|
| |
Protein Sci,
8,
13-24.
|
|
|
|
|
|
|
M.M.Prabu,
K.Suguna,
and
M.Vijayan
(1999).
Variability in quaternary association of proteins with the same tertiary fold: a case study and rationalization involving legume lectins.
|
| |
Proteins,
35,
58-69.
|
|
|
|
|
|
|
N.Manoj,
V.R.Srinivas,
and
K.Suguna
(1999).
Structure of basic winged-bean lectin and a comparison with its saccharide-bound form.
|
| |
Acta Crystallogr D Biol Crystallogr,
55,
794-800.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
R.Ravishankar,
K.Suguna,
A.Surolia,
and
M.Vijayan
(1999).
Structures of the complexes of peanut lectin with methyl-beta-galactose and N-acetyllactosamine and a comparative study of carbohydrate binding in Gal/GalNAc-specific legume lectins.
|
| |
Acta Crystallogr D Biol Crystallogr,
55,
1375-1382.
|
|
|
PDB codes:
|
|
|
|
|
|
|
|
J.M.Richardson,
M.J.Milton,
and
S.W.Homans
(1995).
Solution dynamics of the oligosaccharide moiety of ganglioside GM1: comparison of solution conformations with the bound state conformation in association with cholera toxin B-pentamer.
|
| |
J Mol Recognit,
8,
358-362.
|
|
|
|
|
|
|
R.Loris,
F.Casset,
J.Bouckaert,
J.Pletinckx,
M.H.Dao-Thi,
F.Poortmans,
A.Imberty,
S.Perez,
and
L.Wyns
(1994).
The monosaccharide binding site of lentil lectin: an X-ray and molecular modelling study.
|
| |
Glycoconj J,
11,
507-517.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
R.R.Van Eijsden,
B.S.De Pater,
and
J.W.Kijne
(1994).
Mutational analysis of the sugar-binding site of pea lectin.
|
| |
Glycoconj J,
11,
375-380.
|
|
|
|
|
|
|
Y.Bourne,
J.Mazurier,
D.Legrand,
P.Rougé,
J.Montreuil,
G.Spik,
and
C.Cambillau
(1994).
Structures of a legume lectin complexed with the human lactotransferrin N2 fragment, and with an isolated biantennary glycopeptide: role of the fucose moiety.
|
| |
Structure,
2,
209-219.
|
|
|
PDB codes:
|
|
|
|
|
|
|
|
H.Lis,
and
N.Sharon
(1993).
Protein glycosylation. Structural and functional aspects.
|
| |
Eur J Biochem,
218,
1.
|
|
|
|
|
|
|
A.Ayouba,
H.Debray,
and
P.Rougé
(1992).
Fine sugar specificity of the Butea frondosa seed lectin.
|
| |
Glycoconj J,
9,
141-147.
|
|
|
|
|
|
|
A.Imberty,
M.M.Delage,
Y.Bourne,
C.Cambillau,
and
S.Pérez
(1991).
Data bank of three-dimensional structures of disaccharides: Part II, N-acetyllactosaminic type N-glycans. Comparison with the crystal structure of a biantennary octasaccharide.
|
| |
Glycoconj J,
8,
456-483.
|
|
|
|
|
|
|
J.Mazurier,
M.Dauchez,
G.Vergoten,
J.Montreuil,
and
G.Spik
(1991).
Molecular modeling of a disialylated monofucosylated biantennary glycan of the N-acetyllactosamine type.
|
| |
Glycoconj J,
8,
390-399.
|
|
|
|
|
|
The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
|
 |
|
Links
