PDBsum entry 1kuu

Structural genomics, unknown function

PDB id

1kuu

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Contents

			Protein chain

					202 a.a. *

			Waters ×32

* Residue conservation analysis

PDB id:

1kuu

Links

Name:

Structural genomics, unknown function

Title:

Crystal structure of methanobacterium thermoautotrophicum conserved protein mth1020 reveals an ntn-hydrolase fold

Structure:

Conserved protein. Chain: a. Engineered: yes

Source:

Methanothermobacter. Organism_taxid: 145260. Gene: mth1020. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Biol. unit:

Tetramer (from PDB file)

Resolution:

2.20Å

R-factor:

0.229

R-free:

0.248

Authors:

V.Saridakis,D.Christendat,A.Thygesen,C.H.Arrowsmith,A.M.Edwards, E.F.Pai

Key ref:

V.Saridakis et al. (2002). Crystal structure of Methanobacterium thermoautotrophicum conserved protein MTH1020 reveals an NTN-hydrolase fold. Proteins, 48, 141-143. PubMed id: 12012346 DOI: 10.1002/prot.10147

Date:

22-Jan-02

Release date:

29-May-02

PROCHECK

	Headers

	References

Protein chain

O27099 (PURO_METTH) - IMP cyclohydrolase from Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)

Seq: Struc:					202 a.a. 202 a.a.

Key:

PfamA domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class:

E.C.3.5.4.10 - Imp cyclohydrolase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Pathway:

Purine Biosynthesis (late stages)

Reaction:

IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide

IMP	+	H2O	=	5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

DOI no: 10.1002/prot.10147	Proteins 48:141-143 (2002)
PubMed id: 12012346

Crystal structure of Methanobacterium thermoautotrophicum conserved protein MTH1020 reveals an NTN-hydrolase fold.
V.Saridakis, D.Christendat, A.Thygesen, C.H.Arrowsmith, A.M.Edwards, E.F.Pai.

ABSTRACT

Selected figure(s)



	Figure 1. Figure 1. A: Ribbon diagram of a subunit of MTH1020. The protein is composed of a single domain with an - - core. The secondary structure elements are numbered. B: Structure superposition of MTH1020 (green) with a member of the NTN-hydrolase family (blue) showing the similarity of the structure of MTH1020 with NTN-hydrolases. The arrows depict Arg5 and the N-terminal nucleophile of the NTN-hydrolase member in ball and stick form showing the similarity in position of Arg5 with the N-terminal nucleophile. The programs MOLSCRIPT and RASTER 3D were used in the production of the figures.

Literature references that cite this PDB file's key reference

PubMed id

Reference

18712276

Y.Zhang, M.Morar, and S.E.Ealick (2008).
Structural biology of the purine biosynthetic pathway.

Cell Mol Life Sci, 65, 3699-3724.

17407260

Y.N.Kang, A.Tran, R.H.White, and S.E.Ealick (2007).
A novel function for the N-terminal nucleophile hydrolase fold demonstrated by the structure of an archaeal inosine monophosphate cyclohydrolase.

Biochemistry, 46, 5050-5062.

PDB codes:

2ntk 2ntl 2ntm

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.