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PDBsum entry 1kqh
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DOI no:
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Biochemistry
41:3294-3301
(2002)
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PubMed id:
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Solution structures of the cis- and trans-Pro30 isomers of a novel 38-residue toxin from the venom of Hadronyche Infensa sp. that contains a cystine-knot motif within its four disulfide bonds.
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K.J.Rosengren,
D.Wilson,
N.L.Daly,
P.F.Alewood,
D.J.Craik.
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ABSTRACT
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The primary sequence and three-dimensional structure of a novel peptide toxin
isolated from the Australian funnel-web spider Hadronyche infensa sp. is
reported. ACTX-Hi:OB4219 contains 38 amino acids, including eight-cysteine
residues that form four disulfide bonds. The connectivities of these disulfide
bonds were previously unknown but have been unambiguously determined in this
study. Three of these disulfide bonds are arranged in an inhibitor cystine-knot
(ICK) motif, which is observed in a range of other disulfide-rich peptide
toxins. The motif incorporates an embedded ring in the structure formed by two
of the disulfides and their connecting backbone segments penetrated by a third
disulfide bond. Using NMR spectroscopy, we determined that despite the isolation
of a single native homologous product by RP-HPLC, ACTX-Hi:OB4219 possesses two
equally populated conformers in solution. These two conformers were determined
to arise from cis/trans isomerization of the bond preceding Pro30. Full
assignment of the NMR spectra for both conformers allowed for the calculation of
their structures, revealing the presence of a triple-stranded antiparallel beta
sheet consistent with the inhibitor cystine-knot (ICK) motif.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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P.Da Silva,
A.Strzepa,
L.Jouvensal,
I.Rahioui,
F.Gressent,
and
A.F.Delmas
(2009).
A folded and functional synthetic PA1b: an interlocked entomotoxic miniprotein.
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Biopolymers,
92,
436-444.
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N.Fujitani,
T.Kouno,
T.Nakahara,
K.Takaya,
T.Osaki,
S.Kawabata,
M.Mizuguchi,
T.Aizawa,
M.Demura,
S.Nishimura,
and
K.Kawano
(2007).
The solution structure of horseshoe crab antimicrobial peptide tachystatin B with an inhibitory cystine-knot motif.
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J Pept Sci,
13,
269-279.
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PDB codes:
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G.Ferrat,
F.Bosmans,
J.Tytgat,
C.Pimentel,
B.Chagot,
N.Gilles,
T.Nakajima,
H.Darbon,
and
G.Corzo
(2005).
Solution structure of two insect-specific spider toxins and their pharmacological interaction with the insect voltage-gated Na+ channel.
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Proteins,
59,
368-379.
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PDB codes:
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K.J.Rosengren,
U.Göransson,
L.Otvos,
and
D.J.Craik
(2004).
Cyclization of pyrrhocoricin retains structural elements crucial for the antimicrobial activity of the native peptide.
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Biopolymers,
76,
446-458.
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F.Barbault,
C.Landon,
M.Guenneugues,
J.P.Meyer,
V.Schott,
J.L.Dimarcq,
and
F.Vovelle
(2003).
Solution structure of Alo-3: a new knottin-type antifungal peptide from the insect Acrocinus longimanus.
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Biochemistry,
42,
14434-14442.
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PDB code:
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L.Jouvensal,
L.Quillien,
E.Ferrasson,
Y.Rahbé,
J.Guéguen,
and
F.Vovelle
(2003).
PA1b, an insecticidal protein extracted from pea seeds (Pisum sativum): 1H-2-D NMR study and molecular modeling.
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Biochemistry,
42,
11915-11923.
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PDB code:
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
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