PDBsum entry 1jlh

Isomerase

PDB id

1jlh

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Contents

			Protein chain

					556 a.a. *

			Waters ×1289

* Residue conservation analysis

PDB id:

1jlh

Links
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Name:

Isomerase

Title:

Human glucose-6-phosphate isomerase

Structure:

Phosphoglucose isomerase. Chain: a, b, c, d. Synonym: pgi, glucose phosphate isomerase. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Biol. unit:

Dimer (from PQS)

Resolution:

2.10Å

R-factor:

0.197

R-free:

0.237

Authors:

A.T.Cordeiro

Key ref:

A.T.Cordeiro et al. (2003). Crystal structure of human phosphoglucose isomerase and analysis of the initial catalytic steps. Biochim Biophys Acta, 1645, 117-122. PubMed id: 12573240 DOI: 10.1016/S1570-9639(02)00464-8

Date:

16-Jul-01

Release date:

11-Feb-03

PROCHECK

	Headers

	References

Protein chains

P06744 (G6PI_HUMAN) - Glucose-6-phosphate isomerase from Homo sapiens

Seq: Struc:

Seq: Struc:					558 a.a. 556 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)



		Enzyme reactions

Enzyme class:

E.C.5.3.1.9 - glucose-6-phosphate isomerase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate

alpha-D-glucose 6-phosphate	=	beta-D-fructose 6-phosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

DOI no: 10.1016/S1570-9639(02)00464-8	Biochim Biophys Acta 1645:117-122 (2003)
PubMed id: 12573240

Crystal structure of human phosphoglucose isomerase and analysis of the initial catalytic steps.
A.T.Cordeiro, P.H.Godoi, C.H.Silva, R.C.Garratt, G.Oliva, O.H.Thiemann.

ABSTRACT

The second enzyme in the glycolytic pathway, phosphoglucose isomerase (PGI), catalyses an intracellular aldose-ketose isomerization. Here we describe the human recombinant PGI structure (hPGI) solved in the absence of active site ligands. Crystals isomorphous to those previously reported were used to collect a 94% complete data set to a limiting resolution of 2.1 A. From the comparison between the free active site hPGI structure and the available human and rabbit PGI (rPGI) structures, a mechanism for protein initial catalytic steps is proposed. Binding of the phosphate moiety of the substrate to two distinct elements of the active site is responsible for driving a series of structural changes resulting in the polarisation of the active site histidine, priming it for the initial ring-opening step of catalysis.

Literature references that cite this PDB file's key reference

PubMed id

Reference

15716432

T.Hansen, B.Schlichting, M.Felgendreher, and P.Schönheit (2005).
Cupin-type phosphoglucose isomerases (Cupin-PGIs) constitute a novel metal-dependent PGI family representing a convergent line of PGI evolution.

J Bacteriol, 187, 1621-1631.

15206941

A.T.Cordeiro, P.A.Michels, L.F.Delboni, and O.H.Thiemann (2004).
The crystal structure of glucose-6-phosphate isomerase from Leishmania mexicana reveals novel active site features.

Eur J Biochem, 271, 2765-2772.

PDB codes:

1q50 1t10

15103138

A.T.Cordeiro, R.Hardré, P.A.Michels, L.Salmon, L.F.Delboni, and O.H.Thiemann (2004).
Leishmania mexicana mexicana glucose-6-phosphate isomerase: crystallization, molecular-replacement solution and inhibition.

Acta Crystallogr D Biol Crystallogr, 60, 915-919.

14551194

T.Hansen, D.Wendorff, and P.Schönheit (2004).
Bifunctional phosphoglucose/phosphomannose isomerases from the Archaea Aeropyrum pernix and Thermoplasma acidophilum constitute a novel enzyme family within the phosphoglucose isomerase superfamily.

J Biol Chem, 279, 2262-2272.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.