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PDBsum entry 1j5h
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Antibiotic PDB id
1j5h

 

 

 

 

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Contents
Protein chain
113 a.a.
PDB id:
1j5h
Name: Antibiotic
Title: Solution structure of apo-neocarzinostatin
Structure: Apo-neocarzinostatin. Chain: a. Synonym: ncs, mitomalcin, mmc. Engineered: yes
Source: Streptomyces carzinostaticus. Organism_taxid: 1897. Expressed in: escherichia coli. Expression_system_taxid: 562.
NMR struc: 44 models
Authors: M.D.Urbaniak,F.W.Muskett,M.D.Finucane,S.Caddick,D.N.Woolfson
Key ref:
M.D.Urbaniak et al. (2002). Solution structure of a novel chromoprotein derived from apo-neocarzinostatin and a synthetic chromophore. Biochemistry, 41, 11731-11739. PubMed id: 12269815 DOI: 10.1021/bi0262146
Date:
02-May-02     Release date:   11-Sep-02    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P0A3R9  (NCZS_STRCZ) -  Neocarzinostatin from Streptomyces carzinostaticus
Seq:
Struc: 		147 a.a.
113 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 

 
DOI no: 10.1021/bi0262146 Biochemistry 41:11731-11739 (2002)
PubMed id: 12269815  
 
 
Solution structure of a novel chromoprotein derived from apo-neocarzinostatin and a synthetic chromophore.
M.D.Urbaniak, F.W.Muskett, M.D.Finucane, S.Caddick, D.N.Woolfson.
 
  ABSTRACT  
 
The natural complex Neocarzinostatin comprises a labile chromophore noncovalently bound to an 11.2 kDa protein. We present the first high-resolution structure of a novel complex derived from the recombinant apoprotein bound to a non-natural synthetic chromophore. Fluorescence and nuclear magnetic resonance spectroscopy were used to probe the strength and location of binding. Binding occurred in a location similar to that observed for the chromophore in the natural Neocarzinostatin complex, but with a distinct orientation. These results provide structural evidence that the apoprotein can readily accommodate small druglike entities, other than the natural chromophore within its binding cleft. The clinical use of the natural complex described by others, together with the results reported here, suggests potential applications for small molecule binding by apo-Neocarzinostatin.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
21268152 H.W.Chi, Y.C.Chien, C.Y.Liu, C.J.Tseng, Y.J.Lee, J.L.Chan, Y.R.Chu, and D.H.Chin (2011).
Role of steric effects in protein-directed enediyne cycloaromatization of neocarzinostatin.
  Chemistry, 17, 1493-1506.  
20336247 B.Wang, and K.M.Merz (2010).
Importance of loop dynamics in the neocarzinostatin chromophore binding and release mechanisms.
  Phys Chem Chem Phys, 12, 3443-3449.  
20735485 V.T.Hang, T.J.Oh, T.Yamaguchi, and J.K.Sohng (2010).
In vivo characterization of NcsB3 to establish the complete biosynthesis of the naphthoic acid moiety of the neocarzinostatin chromophore.
  FEMS Microbiol Lett, 311, 119-125.  
19463188 A.Shanmuganathan, T.K.Kumar, C.M.Huang, C.Yu, and D.H.Chin (2009).
A superior drug carrier--aponeocarzinostatin in partially unfolded state fully protects the labile antitumor enediyne.
  J Biomed Sci, 16, 48.  
18601891 J.Kandaswamy, P.Hariharan, T.K.Kumar, C.Yu, T.J.Lu, and D.H.Chin (2008).
Is association of labile enediyne chromophore a mutually assured protection for carrier protein?
  Anal Biochem, 381, 18-26.  
18387946 Y.Luo, S.Lin, J.Zhang, H.A.Cooke, S.D.Bruner, and B.Shen (2008).
Regiospecific O-methylation of naphthoic acids catalyzed by NcsB1, an O-methyltransferase involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin.
  J Biol Chem, 283, 14694-14702.  
17451164 J.R.Baker, D.N.Woolfson, F.W.Muskett, R.G.Stoneman, M.D.Urbaniak, and S.Caddick (2007).
Protein-small molecule interactions in neocarzinostatin, the prototypical enediyne chromoprotein antibiotic.
  Chembiochem, 8, 704-717.  
16458518 C.G.Sudhahar, and D.H.Chin (2006).
Aponeocarzinostatin--a superior drug carrier exhibiting unusually high endurance against denaturants.
  Bioorg Med Chem, 14, 3543-3552.  
16567802 P.Hariharan, W.Liang, S.H.Chou, and D.H.Chin (2006).
A new model for ligand release. Role of side chain in gating the enediyne antibiotic.
  J Biol Chem, 281, 16025-16033.  
15640161 H.Takashima, T.Yoshida, T.Ishino, K.Hasuda, T.Ohkubo, and Y.Kobayashi (2005).
Solution NMR structure investigation for releasing mechanism of neocarzinostatin chromophore from the holoprotein.
  J Biol Chem, 280, 11340-11346.
PDB code: 1o5p
15169956 M.Nicaise, M.Valerio-Lepiniec, P.Minard, and M.Desmadril (2004).
Affinity transfer by CDR grafting on a nonimmunoglobulin scaffold.
  Protein Sci, 13, 1882-1891.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

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