PDBsum entry 1j5c

Electron transport

PDB id

1j5c

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Contents

			Protein chain

					98 a.a. *

			Metals

					_CU

* Residue conservation analysis

PDB id:

1j5c

Links
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Name:

Electron transport

Title:

Solution structure of oxidized paramagnetic cu(ii) plastocyanin from synechocystis pcc6803

Structure:

Plastocyanin. Chain: a. Engineered: yes. Mutation: yes

Source:

Synechocystis sp. Pcc 6803. Organism_taxid: 1148. Strain: pcc6803. Expressed in: escherichia coli. Expression_system_taxid: 562.

NMR struc:

35 models

Authors:

I.Bertini,S.Ciurli,A.Dikiy,C.O.Fernandez,C.Luchinat,N.Safarov, S.Shumilin,A.J.Vila

Key ref:

I.Bertini et al. (2001). The first solution structure of a paramagnetic copper(II) protein: the case of oxidized plastocyanin from the cyanobacterium Synechocystis PCC6803. J Am Chem Soc, 123, 2405-2413. PubMed id: 11456890 DOI: 10.1021/ja0033685

Date:

02-Apr-02

Release date:

10-Apr-02

Supersedes:

1i0w

PROCHECK

	Headers

	References

Protein chain

P21697 (PLAS_SYNY3) - Plastocyanin from Synechocystis sp. (strain PCC 6803 / Kazusa)

Seq: Struc:					126 a.a. 98 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)

DOI no: 10.1021/ja0033685	J Am Chem Soc 123:2405-2413 (2001)
PubMed id: 11456890

The first solution structure of a paramagnetic copper(II) protein: the case of oxidized plastocyanin from the cyanobacterium Synechocystis PCC6803.
I.Bertini, S.Ciurli, A.Dikiy, C.O.Fernàndez, C.Luchinat, N.Safarov, S.Shumilin, A.J.Vila.

ABSTRACT

The NMR solution structure of oxidized plastocyanin from the cyanobacterium Synechocystis PCC6803 is here reported. The protein contains paramagnetic copper(II), whose electronic relaxation times are quite unfavorable for NMR solution studies. The structure has been solved on the basis of 1041 meaningful NOESY cross-peaks, 18 1D NOEs, 26 T(1) values, 96 dihedral angle constraints, and 18 H-bonds. The detection of broad hyperfine-shifted signals and their full assignment allowed the identification of the copper(II) ligands and the determination of the Cu-S-C-H dihedral angle for the coordinated cysteine. The global root-mean-square deviation from the mean structure for the solution structure family is 0.72 +/- 0.14 and 1.16 +/- 0.17 A for backbone and heavy atoms, respectively. The structure is overall quite satisfactory and represents a breakthrough, in that it includes paramagnetic copper proteins among the metalloproteins for which solution structures can be afforded. The comparison with the available X-ray structure of a triple mutant is also performed.

Literature references that cite this PDB file's key reference

PubMed id

Reference

21258692

M.Choi, and V.L.Davidson (2011).
Cupredoxins--a study of how proteins may evolve to use metals for bioenergetic processes.

Metallomics, 3, 140-151.

20734113

G.L.Butterfoss, E.F.Derose, S.A.Gabel, L.Perera, J.M.Krahn, G.A.Mueller, X.Zheng, and R.E.London (2010).
Conformational dependence of (13)C shielding and coupling constants for methionine methyl groups.

J Biomol NMR, 48, 31-47.

19146411

L.A.Abriata, G.N.Ledesma, R.Pierattelli, and A.J.Vila (2009).
Electronic structure of the ground and excited states of the Cu(A) site by NMR spectroscopy.

J Am Chem Soc, 131, 1939-1946.

17583552

I.Bertini, I.C.Felli, C.Luchinat, G.Parigi, and R.Pierattelli (2007).
Towards a protocol for solution structure determination of copper(II) proteins: the case of Cu(II)Zn(II) superoxide dismutase.

Chembiochem, 8, 1422-1429.

16446449

D.F.Hansen, and J.J.Led (2006).
Determination of the geometric structure of the metal site in a blue copper protein by paramagnetic NMR.

Proc Natl Acad Sci U S A, 103, 1738-1743.

16214856

E.L.Gross, and I.Rosenberg (2006).
A Brownian dynamics study of the interaction of Phormidium cytochrome f with various cyanobacterial plastocyanins.

Biophys J, 90, 366-380.

16795108

K.Sato, and C.Dennison (2006).
Active site comparison of CoII blue and green nitrite reductases.

Chemistry, 12, 6647-6659.

16477687

M.R.Jensen, D.F.Hansen, U.Ayna, R.Dagil, M.A.Hass, H.E.Christensen, and J.J.Led (2006).
On the use of pseudocontact shifts in the structure determination of metalloproteins.

Magn Reson Chem, 44, 294-301.

15691836

F.Musiani, A.Dikiy, A.Y.Semenov, and S.Ciurli (2005).
Structure of the intermolecular complex between plastocyanin and cytochrome f from spinach.

J Biol Chem, 280, 18833-18841.

PDB code:

1ylb

14517909

D.Monleón, and B.Celda (2003).
Study of electrostatic potential surface distribution of wild-type plastocyanin Synechocystis solution structure determined by homonuclear NMR.

Biopolymers, 70, 212-220.

PDB code:

1m9w

12581200

I.Gelis, N.Katsaros, C.Luchinat, M.Piccioli, and L.Poggi (2003).
A simple protocol to study blue copper proteins by NMR.

Eur J Biochem, 270, 600-609.

11988476

M.Ubbink, J.A.Worrall, G.W.Canters, E.J.Groenen, and M.Huber (2002).
Paramagnetic resonance of biological metal centers.

Annu Rev Biophys Biomol Struct, 31, 393-422.

11509552

I.Bertini, D.A.Bryant, S.Ciurli, A.Dikiy, C.O.Fernández, C.Luchinat, N.Safarov, A.J.Vila, and J.Zhao (2001).
Backbone dynamics of plastocyanin in both oxidation states. Solution structure of the reduced form and comparison with the oxidized state.

J Biol Chem, 276, 47217-47226.

PDB codes:

1jxd 1jxf

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.