PDBsum entry 1j2g

Oxidoreductase

PDB id: 1j2g

Contents

Protein chains

306 a.a. *

Ligands

SO4 ×4

AZA ×4

Waters ×409

* Residue conservation analysis

PDB id:

1j2g

Name:

Oxidoreductase

Title:

Crystal structure of urate oxidase from bacillus sp. Tb-90 co- crystallized with 8-azaxanthine

Structure:

Uricase. Chain: a, b, c, d. Fragment: residues 1-319. Synonym: urate oxidase. Engineered: yes

Source:

Bacillus sp.. Organism_taxid: 36824. Strain: tb-90. Expressed in: escherichia coli. Expression_system_taxid: 562.

Biol. unit:

Tetramer (from PQS)

Resolution:

2.20Å R-factor: 0.182 R-free: 0.218

Authors:

T.Hibi,T.Nago,Y.Nishiya,J.Oda

Key ref:

T.Hibi et al. Crystal structure of urate oxidase from bacillus sp.. To be published, .

Date:

05-Jan-03 Release date: 15-Jun-04

Protein chains

Q45697  (PUCL_BACSB) -  Uric acid degradation bifunctional protein from Bacillus sp. (strain TB-90)

Seq: 502 a.a.

Struc: 306 a.a.

Enzyme reactions

• Enzyme class 1: E.C.1.7.3.3 - factor independent urate hydroxylase.
• Pathway: AMP Catabolism
• Reaction: urate + O2 + H2O = 5-hydroxyisourate + H2O2

urate + O2 + H2O (Bound ligand (Het Group name = AZA) matches with 76.92% similarity) = 5-hydroxyisourate + H2O2

• Cofactor: Copper
• Enzyme class 2: E.C.4.1.1.97 - 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase.
• Reaction: 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate + H⁺ = (S)-allantoin + CO2

5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate (Bound ligand (Het Group name = AZA) matches with 66.67% similarity) + H(+) = (S)-allantoin + CO2

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site