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PDBsum entry 1ikc
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Toxin PDB id
1ikc

 

 

 

 

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Contents
Protein chain
74 a.a. *
* Residue conservation analysis
PDB id:
1ikc
Name: Toxin
Title: Nmr structure of alpha-bungarotoxin
Structure: Long neurotoxin 1. Chain: a. Synonym: alpha-bungarotoxin
Source: Bungarus multicinctus. Many-banded krait. Organism_taxid: 8616
NMR struc: 30 models
Authors: N.Niccolai,O.Spiga,A.Ciutti
Key ref:
M.Scarselli et al. (2002). NMR structure of alpha-bungarotoxin free and bound to a mimotope of the nicotinic acetylcholine receptor. Biochemistry, 41, 1457-1463. PubMed id: 11814338 DOI: 10.1021/bi011012f
Date:
03-May-01     Release date:   16-May-01    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P60615  (3L21A_BUNMU) -  Alpha-bungarotoxin from Bungarus multicinctus
Seq:
Struc: 		95 a.a.
74 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 

 
DOI no: 10.1021/bi011012f Biochemistry 41:1457-1463 (2002)
PubMed id: 11814338  
 
 
NMR structure of alpha-bungarotoxin free and bound to a mimotope of the nicotinic acetylcholine receptor.
M.Scarselli, O.Spiga, A.Ciutti, A.Bernini, L.Bracci, B.Lelli, L.Lozzi, D.Calamandrei, D.Di Maro, S.Klein, N.Niccolai.
 
  ABSTRACT  
 
A combinatorial library approach was used to produce synthetic peptides mimicking the snake neurotoxin binding site of nicotinic receptors. Among the sequences, which inhibited binding of alpha-bungarotoxin to muscle and neuronal nicotinic receptors, HRYYESSLPWYPD, a 14-amino acid peptide with considerably higher toxin-binding affinity than the other synthesized peptides, was selected, and the structure of its complex with the toxin was analyzed by NMR. Comparison of the solution structure of the free toxin and its complex with this peptide indicated that complex formation induced extensive conformational rearrangements mainly at finger II and the carboxy terminus of the protein. The peptidyl residues P10 and Y4 seemed to be critical for peptide folding and complex stability, respectively. The latter residue of the peptide strongly interacted with the protein by entering a small pocket delimited by D30, C33, S34, R36, and V39 toxin side chains.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
16086595 A.O.Samson, J.H.Chill, and J.Anglister (2005).
Two-dimensional measurement of proton T1rho relaxation in unlabeled proteins: mobility changes in alpha-bungarotoxin upon binding of an acetylcholine receptor peptide.
  Biochemistry, 44, 10926-10934.  
15606783 M.Talebzadeh-Farooji, M.Amininasab, M.M.Elmi, H.Naderi-Manesh, and M.N.Sarbolouki (2004).
Solution structure of long neurotoxin NTX-1 from the venom of Naja naja oxiana by 2D-NMR spectroscopy.
  Eur J Biochem, 271, 4950-4957.
PDB code: 1w6b
12885641 H.S.Young, L.G.Herbette, and V.Skita (2003).
Alpha-bungarotoxin binding to acetylcholine receptor membranes studied by low angle X-ray diffraction.
  Biophys J, 85, 943-953.  
12770823 L.Lozzi, B.Lelli, Y.Runci, S.Scali, A.Bernini, C.Falciani, A.Pini, N.Niccolai, P.Neri, and L.Bracci (2003).
Rational design and molecular diversity for the construction of anti-alpha-bungarotoxin antidotes with high affinity and in vivo efficiency.
  Chem Biol, 10, 411-417.  
12900418 S.W.Chi, K.H.Park, J.E.Suk, B.M.Olivera, J.M.McIntosh, and K.H.Han (2003).
Solution conformation of alphaA-conotoxin EIVA, a potent neuromuscular nicotinic acetylcholine receptor antagonist from Conus ermineus.
  J Biol Chem, 278, 42208-42213.
PDB code: 1pqr
12160749 A.Samson, T.Scherf, M.Eisenstein, J.Chill, and J.Anglister (2002).
The mechanism for acetylcholine receptor inhibition by alpha-neurotoxins and species-specific resistance to alpha-bungarotoxin revealed by NMR.
  Neuron, 35, 319-332.
PDB codes: 1l4w 1ljz 1lk1
12006581 F.Teixeira-Clerc, A.Ménez, and P.Kessler (2002).
How do short neurotoxins bind to a muscular-type nicotinic acetylcholine receptor?
  J Biol Chem, 277, 25741-25747.  
12162733 L.Bracci, L.Lozzi, A.Pini, B.Lelli, C.Falciani, N.Niccolai, A.Bernini, A.Spreafico, P.Soldani, and P.Neri (2002).
A branched peptide mimotope of the nicotinic receptor binding site is a potent synthetic antidote against the snake neurotoxin alpha-bungarotoxin.
  Biochemistry, 41, 10194-10199.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

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